2012 Fiscal Year Final Research Report
Explore of the regulatory factors involved in the nitrogen metabolic conversion in the ontogeny of amphibians
| Project/Area Number |
23770061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Toyama |
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Principal Investigator |
KONNO Norifumi 富山大学, 大学院・理工学研究部, 助教 (50507051)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 比較内分泌学 |
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| Research Abstract |
During amphibian metamorphosis, the liver begins to synthesize the urea cycle enzymes necessary to create urea from carbon dioxide and ammonia. We explored the regulatory factor involved in a shift of nitrogen metabolism from ammonotelic to ureotelic. The expression of carbamoyl phosphate synthetase 1 （CPS1) mRNA was first detected at st.47 tadpoles, suggesting that early tadpole produces and excretes urea. We examined the mRNA expression changes in glucocorticoid receptor （GR), CREB, HNF3α, and C/EBPαduring ontogeny. In the results, GR expression was markedly increased in st.44-45 tadpole. We also revealed that corticosterone treatment for Xenopus tadpole （st.44) increased the expression level of CPS I mRNA. Accordingly, CPS I expression （urea synthesis) in early tadpole of Xenopus laevis appears to be mediated by corticosterone and its receptor （GR).
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Research Products
(20 results)
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[Journal Article] The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors2012
Author(s)
Yamaguchi Y, Kaiya H, Konno N, Iwata E, Miyazato M, Uchiyama M, Bell JD, Toop T, Donald JA, Brenner S, Venkatesh B,Hyodo S.
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Journal Title
General and Comparative Endocrinology
Volume: 178
Pages: 519-528.
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[Journal Article] Uchiyama M, Maejima S, Yoshie S, Kubo Y, Konno N, Joss J2012
Author(s)
Uchiyama M, Maejima S, Yoshie S, Kubo Y, Konno N, Joss J
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Journal Title
Proceedings of the Royal Society B
Volume: 279
Pages: 4795-4802.
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[Journal Article] Neuropeptide Y in tiger puffer (Takifugu rubripes): distribution, cloning, characterization and mRNA expression responses to prandial condition.2011
Author(s)
Kamijo M, Kojima K, Maruyama K, KonnoN, Motohashi E, Ikegami T, Uchiyama M,Shioda S, Ando H, Matsuda K
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Journal Title
Zoological Science
Volume: 28
Pages: 882-890
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