2012 Fiscal Year Final Research Report
Study of human HLTF, a ubiquitin ligase for PCNA under DNA damage
| Project/Area Number |
23770125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
HISHIKI Asami 横浜市立大学, 大学院・生命ナノシステム科学研究科, 特任助教 (60571172)
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| Project Period (FY) |
2011 – 2012
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| Keywords | X線結晶解析 |
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| Research Abstract |
Helicase-Like Transcription Factor (HLTF) is involved in template switching pathway, one of DNA damage tolerance mechanism, which allows continuous DNA synthesis even in the presence of DNA lesion. HLTF consists of RING, Snf2, and HIRAN domains. RING and Snf2 domains are responsible for ubiquitination of PCNA and unwinding of DNA, respectively. The N-terminal HIRAN domain is predicted as a novel DNA-binding domain. We tried to prepare human HLTF (hHLTF) RING and HIRAN domains as recombinant proteins. We analyzed interaction between hHLTF HIRAN domain and DNA by Electrophoresis Mobility Shift Assay.
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[Journal Article] Idenification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication2012
Author(s)
Punchihewa C, Inoue A, Hishiki A, Fujikawa Y, Connelly M, Evison B, Shao Y, Heath R, Kuraoka I, Rodrigues P, Hashimoto H, Kawanishi M, Sato M, Yagi T, Fujii N
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Journal Title
J Biol Chem
Volume: Vol. 287
Pages: 14289-14300
DOI
Peer Reviewed
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