2012 Fiscal Year Final Research Report
Structural biology of the vertebrate kinetochore complex CENP-T/W and CENP-S/X complexes
| Project/Area Number |
23770128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
NISHINO Tatsuya 国立遺伝学研究所, 分子遺伝研究系, 助教 (50533155)
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| Project Period (FY) |
2011 – 2012
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| Keywords | X 線結晶解析 |
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| Research Abstract |
In eukaryotes, kinetochore is built at the centromeric region of chromosomes and they connect chromosome and microtubules during cell division. To understand the molecular mechanisms, we analyzed CENP-SX and CENP-TW complexes. Both were similar to histones but they were more similar to each other. They formed a stable CENP-TWSX tetramer. Biochemical analysis of CENP-TWSX tetramer revealed that its DNA binding property is different from that of CENP-TW or CENP-SX. These analyses suggest that CENP-TWSX complex forms a unique chromatin at eukaryotic centromere
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