Metal enhanced effect for stable single molecule imaging

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Osaka University (2012-2013); Hokkaido University (2011)
• Principal Investigator: ARAI Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
• Project Period (FY): 2011 – 2012
• Keywords: １分子 / 金属増強効果 / 金属ナノ粒子

Research Abstract

Single molecule imaging is a powerful technique for the direct observation of dynamics of biomolecules. However, photo-bleaching and signal fluctuations of fluorophores prevent long time observation. To overcome this problem, here, we made stable single molecule observation system by using nano-particle metal enhancement effect for fluorescent probes. We could observe signal enhancement of single molecule fluorescence by Ag nano-particles with Au as core. In addition, by coating SiO2 on Ag nano-particles coated glass surface, we could maintain the mammalian cell state and could observe metal enhanced fluorescent signals of fluorescently labeled epidermal growth factor.

Research Products

• [Journal Article] Statistical characterisation of single-stranded DNA motion near glass surface beyond diffusion coefficient (2014)
  • Author(s): Uehara, S, Hanasaki, I, Arai, Y, Nagai, T, Kawano, S.
  • Journal Title: Micro and Nano Letters Volume: 9 Pages: 257-260
  • DOI: 10.1049/mnl.2013.0668
  • Peer Reviewed
• [Journal Article] Rapid Severing and Motility of Chloroplast-Actin Filaments Are Required for the Chloroplast Avoidance Response in Arabidopsis (2014)
  • Author(s): Kong, S.-G., Arai, Y., Suetsugu, N., Yanagida, T., and Wada, M.
  • Journal Title: Plant Cell Volume: 25 Pages: 572–590
  • DOI: 10.1105/tpc.113.109694
  • Peer Reviewed
• [Journal Article] Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement (2013)
  • Author(s): Yamada, M., Kumamoto, K., Mikuni, S., Arai, Y., Kinjo, M., Nagai, T., Tsukasaki, Y., Watanabe, T.M., Fukui, M., Jin, M., et al.
  • Journal Title: Nature Communications Volume: 4 Pages: 1–10
  • DOI: 10.1038/ncomms3033
  • Peer Reviewed
• [Journal Article] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance (2013)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Mehmood, R., Kimoto, C., Fujita, T., Touma, K., Kaneko, A., Kamikawa, Y., Moriyama, T., et al.
  • Journal Title: Developemental Cell Volume: 26 Pages: 123–135
  • DOI: 10.1016/j.devcel.2013.06.022
  • Peer Reviewed
• [Journal Article] Simultaneous Observation of the Lever Arm and Head Explains Myosin VI Dual Function (2012)
  • Author(s): Ikezaki, K., Komori, T., Sugawa, M., Arai, Y., Nishikawa, S., Iwane, A.H., and Yanagida, T.
  • Journal Title: Small Volume: 8 Pages: 3035–3040
  • DOI: 10.1002/smll.201200765
  • Peer Reviewed
• [Presentation] Fast single molecule particle tracking and analysis plugin with Java Native Interface (2012)
  • Author(s): Arai Y.
  • Organizer: ImageJ User and Developer Conference 2012
  • Place of Presentation: Center de Recherche Public Henri Tudor, Luxembourg
  • Year and Date: 20121024-26
• [Remarks] 1分子輝点追跡ソフトウェアParticle Track and Analysis
  • URL: http://www.sanken.osaka-u.ac.jp/labs/bse/ImageJcontents/frameImageJ.html
• [Remarks] 新学術領域「少数性生物学」第1回トレーニングコース 講師,大阪大学産業科学研究所, 2013年7月28日-8月10日
• [Remarks] 新井由之,輝点自動検出・追跡ツールの開発,「画像科学」夏の勉強会,核融合科学研究所(岐阜), 2012年9月25日-28日(招待講演)