2012 Fiscal Year Final Research Report
Functional and single-molecular analysis of neutrophil in vivo by non-invasive mesurements
| Project/Area Number |
23770171
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIKUSHIMA Kenji 東京大学, 大学院・理学系研究科, 研究員 (50569142)
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| Research Collaborator |
MIKAMI Takashi 東京大学, 大学院・理学系研究科, 大学院生
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| Project Period (FY) |
2011 – 2012
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| Keywords | バイオイメージング |
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| Research Abstract |
I created a fluorescent dye enough bright for the non-invasive imaging of mice using quantum dots. I constructed a high-contrast imaging system and succeeded in non-invasive observation of neutrophil labeled by the quantum dots in mouse ear. Fast vesicle movements inside the neutrophils were also able to be observed and were analyzed. I constructed a new microscope that enables us to measure momentum of the particle that has an undetermined form. Using the system, I measured that vesicles in Hela cell were transported with 2-38 pN force.
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