The regulation mechanism of protein biosynthesis on ribosome in the hibernation stage

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770206
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: KATO Takayuki 大阪大学, 生命機能研究科, 助教 (20423155)
• Research Collaborator: MIYATA Tomoko 大阪大学, 生命機能研究科, 特任助教 (30423156) ; YOSHIDA Hideji 大阪医科大学, 基礎医学, 准教授 (60288735) ; UETA Masami 吉田生物研究所, 研究員 (30512511) ; WADA Akira 吉田生物研究所, 室長 (80025387)
• Project Period (FY): 2011 – 2012
• Keywords: 100S リボソーム / 飢餓的ストレス / 低温電子顕微鏡 / 単粒子解析

Research Abstract

To understand the regulation mechanism of protein biosysnthesis under the starvation stress, structural analysis of 100S ribosomes was carried out by cryo-EM. In the case of E. coli, 30S subunit inserted to the entrance for mRNA. On the other hand, in the case of Staphylococcus Aureus, the exit site for mRNA was covered each 30S. And we confirmed the binding mode of Erviniawhich has E. coli type HPF and Lactobacilluswhich has Staphylococcustype HPF by cryoEM. So we demonstrated that two types of HPF produces two different types of binding mode of 100S ribosome.

Research Products

• [Journal Article] Architecture of a flagellar apparatus in the fast-swimming magnetotactic bacterium MO-1. (2012)
  • Author(s): Ruan, J., Kato, T., Santini, C.-L., Miyata, T., Kawamoto, A., Zhang, W.-J., Bernadac, A., Wu, L.-F. & Namba, K.
  • Journal Title: Proc. Natl Acad. Sci. USA Volume: 109 Pages: 20643-20648
  • DOI: doi:10.1073/pnas.1215274109
  • Peer Reviewed
• [Presentation] Structural analysis of 100S ribosomes in two different types of bacteria. (2013)
  • Author(s): Kato, T., Ueta, M., Miyata, T., Wada, C., Yoshida, H., Wada, A. & Namba, K.
  • Organizer: Nagoya Symposium -Frontiers in Structural Physiology-.
  • Place of Presentation: Nagoya University(愛知県・名古屋市)
  • Year and Date: 20130122-24
• [Presentation] 飢餓的ストレス条件下における細菌の生存戦略 (2012)
  • Author(s): 加藤貴之, 上田雅美, 宮田知子, 吉田秀司, 和田千恵子, 和田明, 難波啓一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県・福岡市)
  • Year and Date: 2012-12-13
• [Presentation] 飢餓的ストレス下での100S リボソーム形成による細菌の生存戦略.Survival strategy ofbacteria under the starvation stress by 100S ribosome formation. (2012)
  • Author(s): Kato, T., Ueta, M., Miyata, T., Yoshica, H., Wada, A., Namba, K.
  • Organizer: 第50回日本生物物理学会年会.
  • Place of Presentation: 名古屋大学(愛知県・名古屋市)
  • Year and Date: 2012-09-24
• [Presentation] Structural analysis of flagella motor by cryoEM (2012)
  • Author(s): Kato, T.
  • Organizer: Joint Seminar Centre for BioImaging Sciences - Structural biology division of Japan EM.
  • Place of Presentation: National University of Singapore (Singapore)
  • Year and Date: 2012-01-12
• [Presentation] 飢餓的ストレス下における細胞の生存戦略 (2011)
  • Author(s): 加藤貴之
  • Organizer: 平成23年度生理学研究所研究会「電子顕微鏡機能イメージングの医学・生物学への応用」
  • Place of Presentation: 岡崎統合バイオサイエンスセンター(愛知県・岡崎市)
  • Year and Date: 20111130-1201
• [Presentation] 飢餓的ストレス下における細菌の生存戦略(The survival strategy in bacteria under the starvation stress). (2011)
  • Author(s): Kato, T., Ueta, M., Miyata, T., Yoshida, H., Maki, Y., Furuike, S., Sakai, A., Wada, A., Namba, K.
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス(兵庫県・姫路市)
  • Year and Date: 2011-09-18
• [Presentation] 低温電子顕微鏡を用いた電子線トモグラフィーによる生体超分子の立体構造解析. The structural analysis of macromolecule by electron cryotomography. (2011)
  • Author(s): 加藤貴之, Ruan Juanfang, 川本晃大, 松尾里紗, 宮田真人, 難波啓一.
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡国際会議場他(福岡県・福岡市)
  • Year and Date: 2011-05-16
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02