2014 Fiscal Year Final Research Report
Cell cycle and organelle specific proteomic analysis of eukaryotic cell
| Project/Area Number |
23770234
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-28 – 2015-03-31
|
| Keywords | 細胞分裂 / 分裂装置 / 細胞小器官 |
|---|
| Outline of Final Research Achievements |
We identified novel kinesin-like protein named TOP from unicellular red alga Cyanidioschyzon merolae. It was revealed that TOP composes a dividing machinery of both chloroplast and mitochondrion and regulates organelle division process. In addition, we discovered the third dividing machinery from the peroxisome of C. merolae and clarified the working mechanism of the machinery. We have established a reliable and reproducible method for gene targeting in C. merolae. Our method will be applicable to the manipulation of genes targeted based on proteomic analysis, i.e. gene disruption and replacement. Technology of data analysis method used in the studies above has been patented.
|
| Free Research Field |
構造生物学
|
