Screening of bifidobacterial genes specifically expressed in the mouse intestine using a site-specific recombination system

2012 Fiscal Year Final Research Report

• Project/Area Number: 23780072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Hokkaido University
• Principal Investigator: FUKIYA Satoru 北海道大学, 大学院・農学研究院, 助教 (10370157)
• Project Period (FY): 2011 – 2012
• Keywords: ビフィズス菌 / 健康増進効果 / 部位特異的組換え / R-IVET / 特異的遺伝子発現

Research Abstract

Bifidobacteria are a major component of the human intestinal microbiota and well-known for their health-promoting effects. However, molecular mechanisms of the effects are still unclear. This study aimed to clarify the mechanisms of the effects by indentifying bifidobacterial genes that are specifically expressed in the intestine. A molecular tool for introduction of the foreign genes to bifidobacterial chromosome was developed. Screening system of the specifically-expressed genes has been constructed based on the developed tool. These progresses are thought to be important for providing evidence at a molecular level for the beneficial effects of bifidobacteria.

Research Products

• [Journal Article] Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. (2012)
  • Author(s): Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.
  • Journal Title: Appl. Environ. Microbiol Volume: 78(14) Pages: 4984-4994
  • Peer Reviewed
• [Journal Article] Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments. (2012)
  • Author(s): Fukiya, S., Hirayama, Y., Sakanaka, M., Kano, Y., Yokota, A.
  • Journal Title: Biosci. Microb. Food. Health Volume: 31(2) Pages: 15-25
  • Peer Reviewed
• [Presentation] プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発 (2013)
  • Author(s): 吹谷智,平山洋佑,横田篤
  • Organizer: 大会シンポジウム4SY24「プロバイオティクスの機能評価と新展開-ヒトの健康と疾病予防を支える乳酸菌とビフィズス菌-」日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城県仙台市)
  • Year and Date: 2013-03-27
• [Presentation] 二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発 (2013)
  • Author(s): 吹谷智,平山洋佑,阪中幹祥,福間英訓,村山寛樹,加納康正,横田篤
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学(滋賀県長浜市)
  • Year and Date: 2013-03-10
• [Presentation] ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析 (2012)
  • Author(s): 阪中幹祥、阿部光紗、平山洋佑、吹谷智、加納康正、横田篤
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学(東京都)
  • Year and Date: 2012-03-10
• [Presentation] Characterization of transposition property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 6th Asian Conference on Lactic Acid Bacteria
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Development of targeted gene disruption technique in Bifidobacterium longum105-A (2011)
  • Author(s): Yosuke Hirayama, Hidenori Fukuma, Hiroki Murayama, Satoru Fukiya, Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin(オランダ)
  • Year and Date: 2011-08-28
• [Presentation] Transposition activity and insertion property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum 105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin(オランダ)
  • Year and Date: 2011-08-28
• [Presentation] Bifidobacterium longum 105-A のα-ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイへの応用 (2011)
  • Author(s): 平山洋佑,平山洋佑、福間英訓、村山寛樹、吹谷智、加納康正、横田篤
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学(大阪府)
  • Year and Date: 2011-07-11
• [Book] In Lactic Acid Bacteria: Current Progress in Advanced Research, Horizon Scientific Press, Norfolk, UK. (2011)
  • Author(s): Fukiya, S., Suzuki, T., Kano, Y., and Yokota, A.
  • Total Pages: 33-51
  • Publisher: Current status of Bifidobacterium gene manipulation technologies.
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/biseibutsu/ja/info.html