2012 Fiscal Year Final Research Report
Is mitochondrial DNA repair mediated by AP endonuclease involved in the pathophysiology of cancer?
| Project/Area Number |
23780286
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMAMORI Tohru 北海道大学, 大学院・獣医学研究科, 准教授 (00512675)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ミトコンドリア / DNA 修復 / AP エンドヌクレアーゼ / がん |
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| Research Abstract |
Mitochondrial AP endonuclease (mtAPE), which is unidentified at present, is assumed to be important for mitochondrial DNA repair. This study was aimed to elucidate the identity and function of mtAPE. In this study, we examined the subcellular localization of AP endonuclease 1, which is one of mtAPE candidates, and observed no APE1 distribution in pure mitochondrial fraction. The enzymatic activity tests revealed that there was some AP endonuclease activity and substantial AP lyase activity in pure mitochondrial fraction. Furthermore, we also found that the molecular weight of this AP lyase was between 30 and 40 kD.
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[Journal Article] A nucleoside anticancer drug,1-(3-C-ethynyl-beta-D-ribo-pentofuran osyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression.2011
Author(s)
Meike S, Yamamori T, Yasui H, Eitaki M, Matsuda A, Morimatsu M, Fukushima M, Yamasaki Y, Inanami O.
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Journal Title
Mol Cancer
Volume: 10(1)
Pages: 92
URL
Peer Reviewed
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