2012 Fiscal Year Final Research Report
Analysis of membrane protease function in fission yeast
| Project/Area Number |
23780347
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Kagawa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 分裂酵母 / ロンボイドプロテアーゼ |
|---|
| Research Abstract |
In eukaryotic cells, functions of Golgi-localized rhomboid proteases (rhomboid) have many unknown aspects. Disruptants for two types of rhomboid proteases, rob1・ and rob2・, showed pleiotropic phenotypes in fission yeast. The rob1・ strain showed sensitivity to drugs, missorting vacuole-type CPY, aberrant colony formation, and so on. The rob2・ strain showed sensitivity to particular antibiotic. We found that one membrane-bound transcription factor (MTF) could not travel to the nucleus from the Golgi membrane in rob1・ strain. Rob1p with mutation for active site also showed the same phenotype. Furthermore, deduced cleavage site of MTF with mutation for P1 position could not be cleaved in wild-type strain.
|
