2012 Fiscal Year Final Research Report
Design of systemic siRNA delivery system based on quantitative analyses of intracellular trafficking
| Project/Area Number |
23790038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
HAYASHI Yasuhiro 北海道大学, 大学院・薬学研究院, 特任助教 (30548178)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 核酸デリバリー / 細胞内動態 / 肝臓 / 急性肝炎 / 2型糖尿病 |
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| Research Abstract |
In this study, comparative analyses between in vitro and in vivo settings were examined in order to develop an efficient in vivo nucleic acid delivery system. We focused on three main intracellular trafficking processes; accumulation, endosome escape, and decondensation process in tissues or cells. We found that the accumulation process is a key factor in achieving in vivo nucleic acid activity at low dose. In addition, nucleic acid activity was increased by improving nucleic acid decondensation process in cytoplasm. Lastly, we have succeeded to prevent acute liver hepatitis and the symptoms of type 2 diabetes and obesity in mice by using the in vivo nucleic acid delivery system.
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[Presentation] An integrated strategywith DNA microarray and non-viral in vivo siRNA delivery to discover novel therapeutic targets for type 2 diabetes2012
Author(s)
Y. Hayashi, K. Kazuaki, Y. Sato, E.Suemitsu, A. Akhter, Y. Sakurai, H. Hatakeyama, M. Hyodo, N. Kaji, Y. Baba, H. Harashima.
Organizer
10th Annual discovery on target.
Place of Presentation
Copley Marriott Hotel, Boston, MA, USA.
Year and Date
20121001-03
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