Development of new lipid raft probe

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MAKINO Asami 独立行政法人理化学研究所, 小林脂質生物学研究室, 特別研究員 (20373368)
• Project Period (FY): 2011 – 2012
• Keywords: 脂質ラフト / コレステロール / スフィンゴミエリン / インフルエンザ

Research Abstract

Lipid rafts are plasma membrane lipid domains enriched in sphingolipids and cholesterol. The size, composition and function of lipid rafts are matters of debate partially because of the lack of an appropriate probe to visualize them in situ. We identified a novel, non-toxic mushroom protein that specifically binds a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol. The purified protein, termed Nakanori, labeled cell surface domains in SM and cholesterol-dependent manner.

Research Products

• [Journal Article] Limonoid compounds inhibit sphingomyelin biosynthesis by preventing CERT protein-dependent extraction of ceramides from the endoplasmic reticulum. (2012)
  • Author(s): Hullin-Matsuda F, Tomishige N, Sakai S, Ishitsuka R, Ishii K, Makino A, Greimel P, Abe M, Laviad EL, Lagarde M, Vidal H, Saito T, Osada H, Hanada K, Futerman AH, Kobayashi T.
  • Journal Title: J Biol Chem. Volume: 287(29) Pages: 24397-411
  • Peer Reviewed
• [Journal Article] A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphat e to the cleavage furrow during cytokinesis. (2012)
  • Author(s): Abe M, Makino A, Hullin-Matsuda F, Kamijo K, Ohno-Iwashita Y, Hanada K, Mizuno H, Miyawaki A, Kobayashi T.
  • Journal Title: Mol Cell Biol. Volume: 32(8) Pages: 1396-407
  • Peer Reviewed
• [Journal Article] Spectroscopic evidence for the unusual stereochemical configuration of an endosome-specific lipid. (2012)
  • Author(s): Tan HH, Makino A, Sudesh K, Greimel P, Kobayashi T.
  • Journal Title: Angew Chem Int Ed Engl. Volume: 51(2) Pages: 533-5
  • Peer Reviewed
• [Presentation] A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphospha te to the cleavage furrow during cytokinesis (2012)
  • Author(s): Abe M, Makino A, Hullin-Matsuda F, Kamijo K, Ohno-Iwashita Y, Hanada K, Mizuno H, Miyawaki A and Kobayashi T
  • Organizer: International Conference on Membrane Domains
  • Place of Presentation: Dijon, France
  • Year and Date: 20121127-30
• [Presentation] A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphospha te to the cleavage furrow during cytokinesis (2012)
  • Author(s): Abe M, Makino A, Hullin-Matsuda F, and Kobayashi T
  • Organizer: Lipid-Protein Interactions in Membranes: Implications for Health and Disease
  • Place of Presentation: Hyderabad, India
  • Year and Date: 20121101-05
• [Presentation] Limonoid Compounds Inhibit Sphingomyelin Biosynthesis by Preventing CERT-dependent Extraction of Ceramides from the Endoplasmic Reticulum (2012)
  • Author(s): Hullin-Matsuda F, Tomishige N, Sakai S, Ishitsuka R, Ishii K, Greimel P, Makino A, Abe M, Laviad E, Hanada K, Futerman A and Kobayashi T.
  • Organizer: EMBO workshop on Molecular Medicine of Sphingolipids
  • Place of Presentation: Ramot, Israel
  • Year and Date: 20121016-21
• [Presentation] Spectroscopic Evidence for the unusual stereochemical configuration of an endosome-specific lipid, bis(monoacylglycero)phosphate (2012)
  • Author(s): Tan H-H, Makino A, Sudesh K, Greimel P, and Kobayashi T
  • Organizer: 53rd International Conference on the Bioscience of Lipids
  • Place of Presentation: Banff Alberta, Canada
  • Year and Date: 20120905-08
• [Presentation] A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphospha te to the cleavage furrow during cytokinesis (2012)
  • Author(s): Abe M, Makino A, Hullin-Matsuda F, Kamijo K, Ohno-Iwashita Y, Hanada K, Mizuno H, Miyawaki A and Kobayashi T
  • Organizer: Gordon Reseach Conference on Glycolipid and Sphingolipid Biology
  • Place of Presentation: Lucca, Italy
  • Year and Date: 20120422-27