2013 Fiscal Year Final Research Report
Evaluation of antitumor effect of tumor-associated macrophage-targeting liposomes
| Project/Area Number |
23790203
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 葉酸受容体 / リポソーム / マクロファージ / ゾレドロン酸 / がん治療 |
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| Research Abstract |
We investigated the possibility of whether depletion of tumor-associated macrophages (TAMs) by zoledronic acid (ZOL) entrapped in folate-linked liposome (FL-ZOL) could inhibit tumor angiogenesis and consequently tumor growth. FL-ZOL showed high cytotoxicity for FR-positive KB and murine macrophage RAW264.7 cells, but not for FR-negative Colon 26 cells, suggested that FL-ZOL was selectively taken up via FR-mediated endocytosis. However, injections of FL-ZOL did not induce antitumor activities for KB and Colon 26 tumor-bearing mice, and had a lethal effect by high toxicity. From these findings, the severe in vivo toxicity of liposomal ZOL limited its utility for in vivo TAM targeting, although FL-ZOL could selectively induce in vitro cytotoxicity via FR.
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