2012 Fiscal Year Final Research Report
Elucidation of the mechanism of VA-RNA-induced innate immune response
| Project/Area Number |
23790215
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
YAMAGUCHI Tomoko 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクト研究員 (50580130)
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| Project Period (FY) |
2011 – 2012
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| Keywords | アデノウイルス |
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| Research Abstract |
We have developed a VA-RNA-deleted Ad(Ad△VR)vector, in which the transcriptional control elements of the VA-RNA-expression were deleted. Although conventional HEK293 cells did not support the propagation of the Ad△VR vectors, HEK293 transformants inducibly expressing VA-RNA I (VR293 cells) with appropriate induction of VA-RNA I expression allowed the propagation of the Ad△VR vector. The Ad△VR vector showed high transduction efficiency comparable to that of the conventional FG-Ad vector in the cultured cells. The Ad△VR vector may be a safer alternative to the FG-Ad vector.
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Research Products
(8 results)
