Regulatory mechanism of administrating directional migration in motile cells by small GTPases family Rac1

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790247
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Mie University
• Principal Investigator: WANG Shujie 三重大学, 大学院・医学系研究科, 助教 (90567926)
• Project Period (FY): 2011 – 2012
• Keywords: 細胞運動 / 形態形成 / 細胞間相互作用

Research Abstract

Major purposes in this research are to reveal the regulatory mechanisms administrating a directional migration in motile cells. At the front of migrating cells, the higher activity of small GTPase Rac1 control the formation of focal adhesions and maintain adhesion sites toward substratum. Furthermore, Tiam1, the GDP/GTP exchange factor for Rac1, participates in polarized cell migration with the PAR complex of PAR3, PAR6, and atypical protein kinase C (aPKC) at adhesion sites. This research shows that Tiam1 interacts directly with talin, which binds and activates integrins to mediate their signaling, and also demonstrates that Tiam1 accumulates at adhesion sites in a dependent on talin and the PAR complex. Furthermore, this research also demonstrates the regulatory mechanisms controlling Rac1 activation and adhesion turnover for the polarized cell migration.

Research Products

• [Journal Article] Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration (2012)
  • Author(s): Wang S, Watanabe T, Matsuzawa K, Katsumi A, Kakeno M, Matsui T, Ye F, Sato K, Murase K, Sugiyama I, Kimura K, Mizoguchi A, Ginsberg MH, Collard JG, Kaibuchi K
  • Journal Title: J. Cell Biol Volume: 199(2) Pages: 331-345
  • Peer Reviewed
• [Journal Article] Distinct distribution and localization of Rho-kinase in mouse epithelial, muscle and neural tissues (2012)
  • Author(s): Iizuka M, Kimura K, Wang S, Kato K, Amano M, Kaibuchi K,Mizoguchi A
  • Journal Title: Cell Struct Funct
  • Peer Reviewed
• [Journal Article] The inositol 5-phosphatase SHIP2 is an effector of RhoA and is involved in cell polarity and migration (2012)
  • Author(s): Kato K, Yazawa T, Taki K, Mori K, Wang S, Nishioka T, Hamaguchi T, Itoh T, Takenawa T, Kataoka C, Matsuura Y, Amano M, Murohara T, Kaibuchi K.
  • Journal Title: Mol Biol Cell Volume: 23(13) Pages: 2593-604
  • Peer Reviewed
• [Journal Article] Numb controls E-cadherin endocytosis through p120 catenin with aPKC. (2011)
  • Author(s): Sato K, Watanabe T, Wang S, Kakeno M, Matsuzawa K, Matsui T, Yokoi K, Murase K, Sugiyama I, Ozawa M, Kaibuchi K.
  • Journal Title: Mol Biol Cell Volume: 22(17 Pages: 3103-19
  • Peer Reviewed
• [Presentation] Tiam1 acts with the PAR complex to control talin-mediated Rac1 activation during polarized cell migration (2011)
  • Author(s): Watanabe T, Wang S, Matsuzawa K, Sato K,Kakeno M, Matsui T, Kaibuchi K
  • Organizer: Gordon Research Conference, Gradient Sensing & Directional Cell Migration
  • Place of Presentation: Switzerland, Les Diablerets, Les Diablerets Conference Center
  • Year and Date: 20110608-09
• [Presentation] Tiam1 acts with the PAR complex to control talin-mediated Rac1 activation during polarized cell migration (2011)
  • Author(s): 渡辺 崇、王 淑杰、松沢 健司、佐藤 和秀、掛布 真愛、松井 利憲、貝淵 弘三
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道, 札幌市, 北海道大学
  • Year and Date: 2011-06-29