2013 Fiscal Year Final Research Report
Molecular mechanisms underlying the glia-mediated osmo-regulation
| Project/Area Number |
23790256
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KAGE-NADAKAI Eriko (鹿毛 枝里子) 東京女子医科大学, 医学部, 講師 (40453790)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 浸透圧 / グリア細胞 / 線虫 |
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| Research Abstract |
We characterized three Caenorhabditis elegansHMIT genes, hmit-1.1, hmit-1.2, and hmit-1.3. hmit-1.1 was expressed in the intestine, and hmit-1.2 was expressed in the glia and the excretory canal, which is an osmotic regulatory organ that is functionally analogous to the kidney. hmit-1.3 was expressed in the intestine and the glia. The expression of hmit-1.1 and hmit-1.2 but not hmit-1.3, was markedly induced under hyperosmotic conditions. Animals with mutant hmit-1.1 and hmit-1.2 were hypersensitive to osmotic stress. The defects of hmit-1.1 and hmit-1.2 mutants were rescued by hmit-1.1 and hmit-1.2 transgenes, respectively, and by modified human HMIT. In human cell lines, HMIT expression was induced in hyperosmotic conditions. These findings indicate that the C. elegans HMIT family has a crucial role in the osmoprotective response.
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[Journal Article] Mitochondria-type GPAT is required for mitochondrial fusion2013
Author(s)
Ohba Y, Sakuragi T, Kage-Nakadai E, Tomioka NH, Kono N, Imae R, Inoue A, Aoki J, Ishihara N, Inoue T, Mitani S, Arai H
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Journal Title
EMBO Journal
Volume: 32
Pages: 1265-1279
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[Journal Article] NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane2012
Author(s)
Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y
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Journal Title
PLoS One
Volume: 7
Pages: e43050
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[Journal Article] GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans2012
Author(s)
Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
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Journal Title
Molecular Biology of the Cell
Volume: 23
Pages: 982-995
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[Journal Article] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division2011
Author(s)
Nomura, K.H., Murata, D., Hayashi, Y., Dejima, K., Mizuguchi, S., Kage-Nakadai, E., Gengyo-Ando, K., Mitani, S., Hirabayashi, Y., Ito, M., Nomura, K
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Journal Title
Glycobiology
Volume: 21
Pages: 834-848
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