2012 Fiscal Year Final Research Report

Molecular mechanism of mineral transport related to cellular reactive oxygen species level

Research Project

Project/Area Number	23790264
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General physiology
Research Institution	Kanazawa University
Principal Investigator	KIKUCHI Akihiro 金沢大学, 医学系, 博士研究員 (90321752)
Project Period (FY)	2011 – 2012
Keywords	活性酸素 / ミネラル / トランスポーター / X線吸収スペクトル / X線吸収スペクトル
Research Abstract	A fine-tuned balance between production and scavenging of reactive oxygen species (ROS) is crucial for living cells. Since minerals (essential trace elements) can be a factorin regulation of ROS level, mechanisms of mineral transport at molecular level are essential for understanding the tuning mechanism of the ROS level in living cells. In this study, techniques for analysis of local structure around mineral using X-ray absorption spectroscopy has been established. Also, micro crystals of selenoprotein P, a selenium transporter, have been obtained. Further crystallographic study will be contributed the finding of scavenging mechanism of ROS by selenium-sites in the protein.

(2 results)