2012 Fiscal Year Final Research Report

Role of T-cell protein tyrosine phosphatase (TCPTP) in the hypothalamus controlling feeding behavior

Research Project

Project/Area Number	23790276
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Environmental physiology (including Physical medicine and Nutritional physiology)
Research Institution	St. Marianna University School of Medicine
Principal Investigator	FUKUSHIMA Atsushi 聖マリアンナ医科大学, 医学部, 助教 (10442716)
Project Period (FY)	2011 – 2012
Keywords	チロシン脱リン酸化酵素 / TCPTP / NPY / POMC / 視床下部
Research Abstract	The purpose of the present study was to show the possible involvement of T-cell protein tyrosine phosphatase（TCPTP） via STAT3 in pro-opiomelanocortin（POMC） neurons in feeding behavior. To this end, we tried to use Cre-Lox recombination to generate POMC neurons specific deletion of TCPTP. However, we came to realize that the mouse strain which TCPTP was selectively knocked out from POMC neurons showed no behavioral abnormality andexhibited normal feeding behavior and glucose torelance. Thus we thought that another hypothalamic neuron was involved and NPY neuron was candidate. We decided to use NPY-mVenus mouse for the analysis. We also examined the regulation of the TCPTP expression by estrogen in chow and high fat fed mice. We found that the TCPTP expression was up regulated by estrogen and high fat diet. We are currently examining these results using NPY-mVenus mouse.