2012 Fiscal Year Final Research Report
Function of Z-DNA structure in Nrf2-dependent gene activation
| Project/Area Number |
23790319
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
MARUYAMA Atsushi 弘前大学, 医学(系)研究科(研究院), 助教 (10431438)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 酸化ストレス / 転写制御 / Nrf2 / Z-DNA / ヘムオキシゲナーゼ‐1 |
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| Research Abstract |
Left-handed double helix of DNA, called Z-DNA, is an alternative structure of DNA and appears to have many diverse roles in nuclear processes such as transcriptional regulation and nucleosome positioning. In this study, I have developed a method for in vivo detection of Z-DNA formation and show Nrf2 activation is associated with Z-DNA formation in the HO-1 gene promoter. The results implicate Nrf2-dependent Z-DNA formation in HO-1 gene transcriptional activation and suggestthe involvement of BRG1 in Z-DNA formation. Human HO-1 gene promoter might be a good model system to understand Z-DNA formation and how Z-DNA forming sequences regulate geneexpression.
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[Journal Article] Nrf2 in bone marrow-derived cells positivelycontributes to the advanced stage of atherosclerotic plaque formation2012
Author(s)
Harada N, Ito K, Hosoya T, Mimura J, Maruyama A, Noguchi N, Yagami KI, Morito N, Takahashi S, Maher JM, Yamamoto M, Itoh K
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Journal Title
Free Radic Biol Med
Volume: 53
Pages: 2256-2262
DOI
Peer Reviewed
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