2012 Fiscal Year Final Research Report
The role of keratan sulfate regulating neural network rearrangement
| Project/Area Number |
23790360
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脊髄損傷 / 神経可塑性 / プロテオグリカン |
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| Research Abstract |
We have revealed that keratan sulfate (KS), which is a kind ofglycosaminoglycan, strongly inhibits the neurite outgrowth as well as chondroitin sulfate (CS). In this study, I aimed the identification of KS/CSPG and the evaluation of the mechanism recognizing KS/CSPG by neurons. KS/CSPGs were isolated from the injured spinal cords and reactive-astrocytes stimulated by the growth factors using column chromatography, and were identified by the shot-gun proteomics method. Phosphacan was mainly identified as the inhibitory KS/CSPG. Interestingly, the inhibitory KS/CSPG isolated from postnatal 1 day rat brain was also identified as phosphacan. Therefore, I suggest that the injured neurons and developing neurons recognize KS/CSPG in the common manner. In addition, the recombinant KS-bearing phaophacan strongly inhibited the neurite outgrowth, and the deletion of KS binding domain of phosphacan ameliorated the neurite outgrowth. From these result, I suggest that neurons strongly recognize keratan sulfate on phosphacan and inhibit neurite outgrowth.
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[Journal Article] Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury2011
Author(s)
Shiro Imagama, Kazuma Sakamoto, Ryoji Tauchi, Ryuichi Shinjo, Tomohiro Ohgomori, Zenya Ito, Haoqian Zhang, Yoshihiro Nishida, Nagamasa Asami, Sawako Takeshita, Nobuo Sugiura, Hideto Watanabe, Toshihide Yamashita, Naoki Ishiguro, Yukihiro Matsuyama, Kenji Kadomatsu
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Journal Title
The Journal of Neuroscience
Volume: 17091-17102
Pages: 17091-17102
Peer Reviewed
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[Journal Article] Minocycline selectively inhibits M1 polarization of microglia
Author(s)
Kazuyoshi Kobayashi, Shiro Imagama, Tomohiro Ohgomori, Kenichi Hirano, Kenji Uchimura, Kazuma Sakamoto, Akihiro Hirakawa, Hideyuki Takeuchi, Akio Suzumura, Naoki Ishiguro, Kenji Kadomatsu
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Journal Title
Cell Death and Disease
Volume: 4
Pages: e525
Peer Reviewed
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[Presentation] Keratan sulfate in neurodegenerative disease2013
Author(s)
Kazuyoshi Kobayashi, Tomohiro Ohgomori, Shiro Imagama, Zenya Ito, Kei Ando, Kenichi Hirano, Tomohiro Matsumoto, Kenji Kadomatsu, Naoki Ishiguro
Organizer
Orthopedics Research Society
Place of Presentation
San Antonio Texas, USA
Year and Date
20130200
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[Presentation] he role of keratan sulfate in experimental autoimmune neuritis2012
Author(s)
Hiroki Matsui, Kenji Kadomatsu, Tomohiro Ohgomori, Takamitsu Natori, Shiro Imagama, Zenya Ito, Kei Ando, Kenichi Hirano, Ryoji Tauchi, Akio Muramoto, Tomohiro Matsumoto, Naoki Ishiguro
Organizer
Orthopedics Research Society
Place of Presentation
San Francisco California, USA
Year and Date
20120200
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