2012 Fiscal Year Final Research Report
Transcriptome analysis of gastrointestinal metastasistissues and the significance of cancer stem cells in metastasis
| Project/Area Number |
23790402
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 胃癌 / 癌幹細胞マーカー / 粘液形質 |
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| Research Abstract |
In the present study, we searched for novel genes associated with tumor metastasis by comprehensive gene expression methods. We immunohistochemically examined expression and distribution of representative cancer stem cells (CSCs) markers ALDH1, CD44 and CD133 in primary tumors and lymph node metastasisof GC. ALDH1 positivity in diffuse-type GC was significantly higher in lymph node metastasis than in the primary tumor. Next, we screened for genes upregulated in GC from microarray and identified the HOXA10 gene. Immunohistochemically, 221 (30%) of 749 GCs were positive for HOXA10, and its expression showed a significant inverse correlation with the depth of invasion and was observed more frequently in the differentiated type of GC. HOXA10 expression was associated with GC with the intestinal mucin phenotype and correlated with CDX2 and MUC2 expression. Furthermore, the prognosis of patients with positive HOXA10 expression was significantly better than in the negative cases. MTT assay and wound healing assay using HOXA10 siRNA indicated that HOXA10 expression inhibits cell growth and motility. These results suggest that expression of HOXA10 may be a key regulator for GC with the intestinal mucin phenotype.
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[Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer2012
Author(s)
Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Oo HZ, Naito Y, Hinoi T, Ohdan H, Yanagihara K, Aoyagi K, Sasaki H and Yasui W
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Journal Title
Cancer Sci
Volume: 103
Pages: 1744-1750
Peer Reviewed
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[Journal Article] Deficiency of claudin-18 causes paracellular H+ leakage, up-regulation of interleukin-1beta, and atrophic gastritis in mice2012
Author(s)
Hayashi D, Tamura A, Tanaka H, Yamazaki Y, Watanabe S, Suzuki K, Suzuki K, Sentani K, Yasui W, Rakugi H, Isaka Y and Tsukita S
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Journal Title
Gastroenterology
Volume: 142
Pages: 292-304
Peer Reviewed
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[Journal Article] Desmocollin 2 is a new immunohistochemical marker indicative of squamous cell carcinoma differentiation in urothelial carcinoma2011
Author(s)
Hayashi T, Sentani K, Oue N, Anami K, Sakamoto N, Ohara S, Teishima J, Noguchi T, Nakayama H, Taniyama, Matsubara A and Yasui W
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Journal Title
Histopathology
Volume: 59
Pages: 710-721
Peer Reviewed
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