Regulation of JC virus late gene expression

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: ORBA Yasuko 北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (60507169)
• Project Period (FY): 2011 – 2013
• Keywords: ＪＣウイルス / RNA / 脳神経疾患

Research Abstract

JC virus(JCV) late transcripts, which produce agnoprotein, VP1, VP2, and VP3, are polycistronic mRNAs and processed by alternative splicing. We found that JCV late transcripts are processed into mature mRNAs using a unique splicing mechanism. Analysis of virus infection using JCV genomes with splice site mutations indicated that the identified splicing mechanism is necessary for efficient production of late proteins and viral infection.

Research Products

• [Journal Article] Viroporin activity of the JC polyomavirus is regulated by interactions with the adaptor protein complex 3 (2013)
  • Author(s): Suzuki T, Orba Y, Makino Y, Okada Y, Sunden Y, Hasegawa H, Hall WW, Sawa H
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110(46) Pages: 18668-18673
  • DOI: 10.1073/pnas.1311457110
  • Peer Reviewed
• [Journal Article] Relationship between methyl CpG binding protein 2 and JC viral proteins (2013)
  • Author(s): Takahashi K, Orba Y, Kimura T, Wang L, Kohsaka S, Tsuda M, Tanino M, Nishihara H, Nagashima K, Sawa H, Tanaka S
  • Journal Title: Jpn J Infect Dis Volume: 66(2) Pages: 126-132
  • DOI: 10.7883/yoken.66.126
  • Peer Reviewed
• [Journal Article] Identification of a novel polyomavirus from vervet monkeys in Zambia (2013)
  • Author(s): Yamaguchi H, Kobayashi S, Ishii A, Ogawa H, Nakamura I, Moonga L, Hang'ombe BM, Mweene AS, Thomas Y, Kimura T, Sawa H, Orba Y
  • Journal Title: J Gen Virol Volume: 94(Pt6) Pages: 1357-1364
  • DOI: 10.1099/vir.0.050740-0
  • Peer Reviewed
• [Journal Article] Role of JC virus agnoprotein in virion formation (2012)
  • Author(s): Suzuki T, Semba S, Sunden Y, Orba Y, Kobayashi S, Nagashima K, Kimura T, Hasegawa H, Sawa H
  • Journal Title: Microbiol Immunol Volume: 56(9) Pages: 639-646
  • DOI: 10.1111/j.1348-0421
  • Peer Reviewed
• [Presentation] JCウイルスT抗原C末端領域の機能解析 (2013)
  • Author(s): 大場靖子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2013-11-10
• [Remarks]
  • URL: http://www.czc.hokudai.ac.jp/index.html