2013 Fiscal Year Final Research Report
Analysis of host invasion mechanisms of Plasmodium sporozoites focusing on rhoptry proteins.
| Project/Area Number |
23790459
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TOKUNAGA Naohito 愛媛大学, 総合科学研究支援センター, 技術員 (30596151)
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| Project Period (FY) |
2011 – 2012
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| Keywords | マラリア原虫 / スポロゾイト / ロプトリー |
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| Research Abstract |
To elucidate the molecular mechanisms of Plasmodium sporozoite invasion of the target cells, we attempted to identify rhoptry proteins in sporozoites. Gene expression, protein expression and localization analyses in sporozoites were performed, then 8 novel sporozoite rhoptry molecules were identified. Since these proteins are also expressed in merozoites, development of sporozoite stage-specific gene silencing system is required for functional analysis. To achieve this purpose, screening for suitable candidate promoters to swap was performed. By swapping the original promoter with the candidate promoter using homologous recombination system, we succeeded to generate sporozoite stage-specific gene silencing transgenic parasites. Using this system, it was indicated that one of the rhoptry proteins is involved in sporozoite salivary gland invasion.
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[Presentation]2013
Author(s)
Naohito Tokunaga, Mamoru Nozaki, Eri Murata, Takafumi Tsuboi, Tomoko Ishino, and Motomi Torii
Organizer
Screening for sporozoite rhoptry proteins in Plasmodium
Place of Presentation
Keystone symposia, New Orleans, US
Year and Date
20130120-25
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[Presentation]2013
Author(s)
Tomoko Ishino, Yuka Sugino, Naohito Tokunaga, Mamoru Nozaki, Takafumi Tsuboi, and Motomi Torii
Organizer
Functional analyses of sporozoite rhoptry proteins by stage-specific gene silencing system in Plasmodium berghei
Place of Presentation
Keystone symposia, New Orleans, USA
Year and Date
20130120-25
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