Analysis of the Shigella.Host defense interaction

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790471
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases (2012); The University of Tokyo (2011)
• Principal Investigator: OGAWA Michinaga 国立感染症研究所, 細菌第一部, 室長 (80361624)
• Project Period (FY): 2011 – 2012
• Keywords: 感染免疫 / 赤痢菌

Research Abstract

We demonstrated that Tecpr1 is an essential component of the WIPI-2-Tecpr1- Atg5-dependent pathway in selective autophagy for Shigella, Salmonella, GAS, aggresomes, and damaged mitochondria. Next we revealed that Shigella deliver a caspase-4-specific inhibitor, OspC3, to antagonize epithelial cell death and promote infection.

Research Products

• [Journal Article] bacterial caspase inhibitor antagonizes inflammatory cell death and promotes epithelial infection. (2013)
  • Author(s): Kobayashi, T., Ogawa, M., Sanada, T., Mimuro, H., Kim, M., Ashida, H., Akakura, R., Yoshida, M., Kawalec, M., Reichhart, J., Mizushima, T. & Sasakawa, C
  • Journal Title: Cell Host Microbe Volume: 13 Pages: 570-583
  • DOI: DOI:10.1016/j.chom.2013.04.012
  • Peer Reviewed
• [Journal Article] Shigella Targets Epithelial Tricellular Junctions and Uses a Noncanonical Clathrin-Dependent Endocytic Pathway to Spread Between Cells. (2012)
  • Author(s): Fukumatsu, M., Ogawa, M., Arakawa, S., Suzuki, M., Nakayama, K., Shimizu, S., Kim, M., Mimuro, H. & Sasakawa, C
  • Journal Title: Cell Host Microbe Volume: 11 Pages: 325-336
  • DOI: DOI:10.1016/j.chom.2012.03.001.
  • Peer Reviewed
• [Journal Article] The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response. (2012)
  • Author(s): Suzuki, M., Ogawa, M., Oyama, A., Ashida, H., Kobayashi, T., Koyama, T., Nagai, S., Shibata, Y., Gohda, J., Inoue, J., Mizushima, T. & Sasakawa, C
  • Journal Title: Nature Volume: 483 Pages: 623-626
  • DOI: DOI:10.1038/nature10894
  • Peer Reviewed
• [Journal Article] A bacterial effector targets the TRAF6-NFκB pathway to modulate the acute inflammatory response to bacterial invasion of epithelial cells. (2012)
  • Author(s): Sanada, T., Kim, M., Mimuro, H., Ashida, H., Ogawa, M., Mizushima, T., Sasakawa, C
  • Journal Title: Virulence Volume: 3 Pages: 518-520
  • DOI: DOI:10.4161/viru.21451
  • Peer Reviewed
• [Journal Article] Uptake of Shigella-containing pseudopodia by neighboring epithelial cells at tricellular junctions via non-canonical clathrin-dependent trafficking pathway. (2012)
  • Author(s): Fukumatsu, M., Ogawa, M., Kim, M., Mimuro, H., Sasakawa, C
  • Journal Title: Virulence Volume: 3 Pages: 515-517
  • DOI: DOI:10.4161/viru.21740
  • Peer Reviewed
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy. (2012)
  • Author(s): Klionsky, DJ. et al.
  • Journal Title: Autophagy Volume: 8 Pages: 445-544
  • DOI: DOI:10.4161/auto.19496
  • Peer Reviewed
• [Journal Article] 病原細菌を標的とした選択的オートファジーの分子メカニズム (2012)
  • Author(s): 小川道永
  • Journal Title: 生物工学学会誌 Volume: 90 Pages: 200
  • URL: https://www.sbj.or.jp/wp-content/uploads/file/sbj/9004/9004_biomedia_4.pdf
• [Journal Article] 病原細菌を標的としたオートファジーにおける新規認識機構 (2012)
  • Author(s): 小川道永
  • Journal Title: 化学と生物 Volume: 50 Pages: 160-162
  • URL: http://www.jsbba.or.jp/pub/journal_kasei/kasei_contents/vol50_3_2012.html
• [Journal Article] 細胞侵入性細菌のオートファジーによる認識機構 (2012)
  • Author(s): 小川道永
  • Journal Title: 炎症と免疫 Volume: 20 Pages: 151-156
• [Journal Article] A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens. (2011)
  • Author(s): Ogawa, M., Yoshikawa, Y., Kobayashi, T., Mimuro, H., Fukumatsu, M., Kiga, K., Piao, Z., Ashida, H., Yoshida, M., Kakuta, S., Koyama, T., Goto, Y., Nagatake, T., Nagai, S., Kiyono, H., Kawalec, M., Reichhart, J. M. & Sasakawa, C
  • Journal Title: Cell Host Microbe Volume: 9 Pages: 376-389
  • DOI: DOI:10.1016/j.chom.2011.04.010
  • Peer Reviewed
• [Journal Article] Autophagy targeting of Listeria monocytogenes and the bacterial countermeasure. (2011)
  • Author(s): Ogawa, M., Yoshikawa, Y., Mimuro, H., Hain, T., Chakraborty, T. & Sasakawa, C
  • Journal Title: Autophagy Volume: 7 Pages: 310-314
  • DOI: DOI:10.4161/auto.7.3.14581
  • Peer Reviewed
• [Journal Article] Manipulation of autophagy by bacteria for their own benefit. (2011)
  • Author(s): Ogawa, M., Mimuro, H., Yoshikawa, Y., Ashida, H. & Sasakawa, C
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 459-471
  • DOI: DOI:10.1111/j.1348-0421.2011.00343.x
  • Peer Reviewed
• [Journal Article] The role of Tecpr1 in selective autophagy as a cargo receptor. (2011)
  • Author(s): Ogawa, M. & Sasakawa, C
  • Journal Title: Autophagy Volume: 7 Pages: 1389-91
  • DOI: DOI:10.4161/auto.7.11.17151
  • Peer Reviewed
• [Journal Article] Bacteria and host interactions in the gut epithelial barrier. (2011)
  • Author(s): Ashida, H., Ogawa, M., Kim, M., Mimuro, H. & Sasakawa, C
  • Journal Title: Nat chem Biol Volume: 8 Pages: 36-45
  • DOI: DOI:10.1038/nchembio.741
  • Peer Reviewed
• [Journal Article] Host-pathogen interactions: Cell death and infection: A double-edged sword for host and pathogen survival. (2011)
  • Author(s): Ashida, H., Mimuro, H, Ogawa, M., Kobayashi, T., Sanada, T., Kim, M. & Sasakawa, C
  • Journal Title: J Cell Biol Volume: 195 Pages: 931-42
  • DOI: DOI:10.1083/jcb.201108081
  • Peer Reviewed
• [Journal Article] Shigella are versatile mucosal pathogens that circumvent the host innate immune system. (2011)
  • Author(s): Ashida, H., Ogawa, M., Mimuro, H., Kobayashi, T., Sanada, T. & Sasakawa, C
  • Journal Title: Curr Opin Immunol Volume: 23 Pages: 448-55
  • DOI: DOI:10.1016/j.coi.2011.06.001
  • Peer Reviewed
• [Journal Article] 赤痢菌の自然免疫回避機構 (2011)
  • Author(s): 小川道永、笹川千尋
  • Journal Title: 臨床免疫・アレルギー科 Volume: 56 Pages: 600-608
  • URL: http://www.kahyo.com/item/M201111-565
• [Presentation] 赤痢菌の感染戦略～宿主細胞の感染性細胞死に抗うカスパーゼ阻害エフェクター～ (2013)
  • Author(s): 小林泰良,小川道永,真田貴人,三室仁美,笹川千尋
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉(幕張)
  • Year and Date: 2013-03-19
• [Presentation] Tecpr1 による選択的オートファジー認識機構 (2013)
  • Author(s): 小川道永
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉(幕張)
  • Year and Date: 2013-03-18
• [Presentation] Selective autophagy for intracellular bacteria (2012)
  • Author(s): 小川道永
  • Organizer: 11th Korea-Japan International Symposium on Microbiology (XI-KJISM)
  • Place of Presentation: Buyeo, Korea
  • Year and Date: 20120913-14
• [Presentation] 病原細菌と宿主との攻防～感染生物学のススメ～ (2012)
  • Author(s): 小川道永
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-13
• [Presentation] 赤痢菌の感染戦略～宿主細胞の炎症性細胞死に抗うカスパーゼ阻害エフェクター～ (2012)
  • Author(s): 小林泰良,小川道永,三室仁美,笹川千尋
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-13
• [Presentation] 赤痢菌III型分泌OspIによる炎症抑制機構の新規メカニズムの解明 (2012)
  • Author(s): 真田貴人、KimMinsoo、三室仁美、鈴木仁人、小川道永、小山晃穂、芦田浩、小林泰良、小山智洋、長井伸也、柴田佑里、合田仁、井上純一郎、水島恒裕、笹川千尋
  • Organizer: 第95回日本細菌学会関東支部総会
  • Place of Presentation: 東京(お台場)
  • Year and Date: 2012-10-11
• [Presentation] 赤痢菌の上皮細胞内生存戦略としてのパイロプトーシス抑制機構 (2012)
  • Author(s): 小林泰良、小川道永、三室仁美、笹川千尋
  • Organizer: 第95回日本細菌学会関東支部総会
  • Place of Presentation: 東京(お台場)
  • Year and Date: 2012-10-11
• [Presentation] A bacterial effector hijacks a novel Caspase-4 dependent epithelial cell death pathway (2012)
  • Author(s): 小林泰良、小川道永、笹川千尋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-28
• [Presentation] A bacterial effector deamidates Ubc13 to dampen the inflammatory response (2012)
  • Author(s): 真田貴人、キムミンス、三室仁美、鈴木仁人、小山明穂、芦田浩、小川道永、井上純一郎、水島恒裕、笹川千尋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-28
• [Presentation] Intercellular spreading of Shigella flexneri in mammalian cells (2012)
  • Author(s): 福松真、小川道永、三室仁美、笹川千尋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-28
• [Presentation] Tecpr1 in Shigella-selective autophagy (2012)
  • Author(s): 小川道永、笹川千尋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] Autophagy and bacterial infection (2011)
  • Author(s): 小川道永
  • Organizer: 2^<nd> International symposium, Microbes For Health
  • Place of Presentation: パリ
  • Year and Date: 2011-12-01
• [Presentation] 病原細菌としたオートファジー認識機構と病原細菌によるその回避機構 (2011)
  • Author(s): 小川道永
  • Organizer: 第94回細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] 赤痢菌のタイプIIIエフェクターによるパイロプトーシス回避機構 (2011)
  • Author(s): 小林泰良、小川道永、笹川千尋
  • Organizer: 第94回細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-06
• [Presentation] 赤痢菌における細胞間拡散機構 (2011)
  • Author(s): 福松真、小川道永、三室仁美、笹川千尋
  • Organizer: 第94回細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-06
• [Presentation] Shigella flexneri hijack Caspse-4 to evade Pyroptosis (2011)
  • Author(s): 小林泰良、小川道永、笹川千尋
  • Organizer: IMSUT/RCAST . Chiba University Global COE Joint Retreat 2011
  • Place of Presentation: 大磯
  • Year and Date: 2011-09-17
• [Presentation] Intercellular spreading of Shigella flexneri in mammalian cells (2011)
  • Author(s): 福松真、小川道永、三室仁美、笹川千尋
  • Organizer: IMSUT/RCAST . Chiba University Global COE Joint Retreat 2011
  • Place of Presentation: 大磯
  • Year and Date: 2011-09-17
• [Presentation] Autophagy and bacterial infection (2011)
  • Author(s): 小川道永
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-08
• [Presentation] The role of Tecpr1-depedent Selective autophagic pathway in targeting bacterial pathogens (2011)
  • Author(s): 小川道永、笹川千尋
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-08
• [Presentation] Intercellular spreading of Shigella flexneri in mammalian cells (2011)
  • Author(s): 福松真、小川道永、鈴木仁人、三室仁美、笹川千尋
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-08
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/2966