Comprehensive screening and identification of novel molecules involved in the spatiotemoral regulation of endosomes in plasmacytoid dendritic cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790535
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: KITAWAKI Toshio 京都大学, 医学(系)研究科(研究院), 助教 (50378684)
• Project Period (FY): 2011 – 2012
• Keywords: 自然免疫 / 形質細胞様樹状細胞 / I型インターフェロン / 二重レポーターシステム / エンドソーム

Research Abstract

Plasmacytoid dendritic cells (pDC) rapidly produce vast amounts of type I interferon in response to nucleic acids. They play an important role in host defense by responding to pathogen-derived nucleic acids. However, they are also involved in the pathogenesis of immune disorders by responding to self-cell-derived nucleic acids. In this project, we performed our research to clarify mechanisms of type I interferon production by pDC, using a human pDC cell line established in our laboratory, by introducing it a dual reporter system that visualize activation dynamics of endosomes.