Elucidation of new reactivation mechanisms of influenza virus specific memory B cells in mouse model for influenza infection

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: ONODERA Taishi 国立感染症研究所, 免疫部, 主任研究官 (90513143)
• Project Period (FY): 2011 – 2012
• Keywords: 記憶B細胞 / Toll様受容体 / ワクチン / インフルエンザウイルス

Research Abstract

Rapid production of high affinity antibodies by memory B cells, which are generated by prior vaccination and/or infection, plays an important role to improve mortality and morbidity in acute infectious diseases as influenza virus infection. In this study, we showed that memory B cells could produce high neutralizing antibodies against influenza virus more rapidly in CD4+ helper T cells independent way. Moreover, this reactivation mechanism was totally dependent upon B-cell intrinsic TLR7-MyD88 signaling, which is elicited by ssRNA included in virus particles. These findings provide insight into the new reactivating mechanisms of virus-specific memory B cells, together the development of new vaccine strategy to preferentially elicit high affinity antibodies from pre-existing memory B cell pool by booster vaccination.

Research Products

• [Journal Article] Memory B cells in the lung participate in protective humoral immune responses to pulmonary influenza virus reinfection. (2012)
  • Author(s): Taishi Onodera, Yoshimasa Takahashi, Yusuke Yokoi, Manabu Ato, Yuichi Kodama, Satoshi Hachimura, Tomohiro Kurosaki, Kazuo Kobayashi
  • Journal Title: PNAS Volume: 109(7) Pages: 2485-90
• [Journal Article] Primary and Secondary B-Cell Responses to Pulmonary influenza infection. (2012)
  • Author(s): Yoshimasa Takahashi, Taishi Onodera, Kazuo Kobayashi, Tomohiro Kurosaki
  • Journal Title: Infectious Disorders Drug Targets Volume: 12 Pages: 232-40
• [Presentation] B-cell intrinsic Toll-like receptor signaling accelerates memory B cell response to booster influenza Vaccination (2013)
  • Author(s): Taishi Onodera, Tomohiro Kurosaki, Kazuo, Kobayashi, Yoshimasa Takahashi
  • Organizer: Keystone Symposia B cell development and function
  • Place of Presentation: USA
  • Year and Date: 20130210-15
• [Presentation] B-cell intrinsic Toll-like receptor signaling accelerates memory B cell response to booster influenza Vaccination (2012)
  • Author(s): Taishi Onodera, Tomohiro Kurosaki, Kazuo, Kobayashi, Yoshimasa Takahashi
  • Organizer: The 41^<th> JSI annual meeting
  • Place of Presentation: Kobe
  • Year and Date: 2012-05-07
• [Presentation] Memory B cells in the lung participate in protective humoral immune responses to pulmonary influenza virus reinfection (2012)
  • Author(s): Takahashi, Y.Onodera, T., Yokoi, Y., Ato, M.,Hachimura, S., Kurosaki, T., Kobayashi, K
  • Organizer: Keystone symposia, Viral Immunity and Host Gene Influence
  • Place of Presentation: Colorado, USA
  • Year and Date: 2012-03-25
• [Presentation] Role of Toll-like receptor signaling for the development and reactivation of virus-specific memory B cells (2011)
  • Author(s): ONODERA Taishi, AIZAWA Ryutaro, HOSONO Akira, KAMINOGAWA Shuichi, KOBAYASHI Kazuo, TAKAHASHI Yoshimasa
  • Organizer: The 40^<th> JSI annual meeting
  • Place of Presentation: Chiba
  • Year and Date: 20111127-29
• [Presentation] Protective memory B cell responses to influenza virus infection (2011)
  • Author(s): Takahashi, Y., Onodera, T., Yokoi, Y., Kurosaki, T., Kobayashi, K.
  • Organizer: The 40^<th> JSI annual meeting
  • Place of Presentation: Chiba
  • Year and Date: 20111127-29
• [Book] B細胞内因性TLRシグナルによるB細胞応答の制御機構、臨床免疫・アレルギー科58巻 (2012)
  • Author(s): 小野寺大志、高橋宜聖、小林和夫
  • Total Pages: 275-282