Development of periodontal diagnosis system using surface plasmon resonance

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790626
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Meikai University
• Principal Investigator: ONISHI Hidetomo 明海大学, 歯学部・口腔生物再生医工学講座歯周病学分野, 助教 (30580279)
• Project Period (FY): 2011 – 2012
• Keywords: 表面プラズモン共鳴 / バイオセンサ / 歯周病診断

Research Abstract

In this project, system for detecting virulence factors of periodontopathicbacteria by using optical fiber surface plasmon resonance sensor (optical fiber SPR sensor) has been developed. We used o uter membrane protein A (OmpA) a s scaffo ld protein possessed binding domain for IgG at its constant region . Consequently, we successfully created a standard curve that is required for quantification of FDF.

Research Products

• [Journal Article] Modification of forsythia detaching factor by gingival crevicular fluid in periodontitis (2013)
  • Author(s): Onishi H, Ro M, Hayashi J, Tatsumi J, Namba S, Yatabe K, Arakawa S, Izumi Y , Shin K
  • Journal Title: rchives of oral biology Volume: 58 Pages: 1007-1013
  • Peer Reviewed
• [Journal Article] 不織布フィルター法で採取したイヌ末梢血由来単核球とss-リン酸三カルシウム微細顆粒のイヌにおける実験的骨欠損部への骨再生効果 (2012)
  • Author(s): 石井麻紀子,辰巳順一,小野裕貴,小川洋一,遠藤 学,大西英知,大塚秀春,林 丈一朗, 申 基吉吉
  • Journal Title: 明海歯科医学 Volume: 第41巻 2号 Pages: 67-80
  • Peer Reviewed
• [Presentation] 光線力学療法を用いたインプラント周囲炎治療の臨床研究 (2012)
  • Author(s): 成田宗隆,辰巳順一,寺西麻里奈,市村光, 大西英知,谷田部一大,申 基吉吉
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-09
• [Presentation] Cleavage of Forsythia detaching factor by gingival crevicular fluid (2012)
  • Author(s): Onishi H, Nakajima T, Ro M, Ono Y, Hayashi K, Gon H, Yatabe K, Arakawa S, Izumi Y, Shin K
  • Organizer: The98th Annual Meeting of American Academy of Periodontology
  • Place of Presentation: Los Angels,USA
  • Year and Date: 2012-09-29
• [Presentation] 光ファイバ表面プラズモンセンサを用いた歯周病原細菌由来病原因子の定量法の構築 (2012)
  • Author(s): 大西英知,呂 宗彦,小野裕貴,林 鋼兵,寺西麻里奈,西村将吾,石井麻紀子,谷田部一大,申 基吉吉
  • Organizer: 第1 報. 第55回秋季日本歯周病学会学術大会
  • Place of Presentation: 茨城
  • Year and Date: 2012-09-23
• [Presentation] 表面プラズモン共鳴光ファイババイオセンサを利用した抗Forsythia Detaching Factor 抗体の検出に関する研究 (2012)
  • Author(s): 大西英知
  • Organizer: 第17回明海歯科医学会学術大会
  • Place of Presentation: 埼玉
  • Year and Date: 2012-06-07
• [Presentation] 歯周炎患者歯肉溝滲出液によるTannerella forsythia Forsythia Detaching Factor の分子量変化 (2012)
  • Author(s): 呂宗彦,大西英知,林 丈一朗,荒川真一,中島琢磨,和泉雄一,申 基吉吉
  • Organizer: 第55回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-05-18
• [Presentation] Clinical Evaluations of Short Implants in Patients with Periodontal Diseases (2012)
  • Author(s): Hideharu OTSUKA, Koichiro MIKAMI, Satomi NAMBA, Yuki ONO, Munehiko RO, Miki MARUYAMA, Munetaka NARITA, Hidetomo ONISHI , Kazuhiro YATABE, Joichiro HAYASHI, Junichi TATSUMI, and Kitetsu SHIN
  • Organizer: Japan-China Dental Conference 2012
  • Place of Presentation: 成都, 中国
  • Year and Date: 2012-04-26
• [Remarks]
  • URL: http://www.meikai.ac.jp/01about/catetemp2/2011-0325-2036-1.html