2012 Fiscal Year Final Research Report
Relationship between surface potential of nanoparticle andtranslocation to brain via olfactory nerve
| Project/Area Number |
23790659
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUI Yasuto 京都大学, 工学研究科, 講師 (50533501)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ナノ材料 / 衛生 / 細胞・組織 / 脳・神経 / ナノバイオ |
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| Research Abstract |
Recent studies have shown that cellular uptake of nanoparticles depends on diameter, superficial area or surface modification. In this study, we examined that amounts of cellular uptake rely on it by measuring their zeta potential. We suspended 4 different surface modification fluorescent particles and measured their zeta potential and administered each of them to A549 cells, and employed confocal scanning laser microscopy. After thirty minute added particles to cells, we washed these cells three times and detected fluorescence by flow cytometer. Almost all the carboxylate particles invaded a cell and about 60 % aminated them also invaded. On the other hand, few non-modification particles were taken up.
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