2012 Fiscal Year Final Research Report
Study of clinical implication of a novel G protein-coupled receptor GPR119: its possible role in glucose homeostasis
| Project/Area Number |
23791030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
ODORI Shinji 京都大学, 医学研究科, 客員研究員 (30402879)
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| Project Period (FY) |
2011 – 2012
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| Keywords | GPR119 / エンドカンナビノイド / 内因性脂質 / 摂食調節 / 糖代謝調節 |
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| Research Abstract |
To assess clinical implications of GPR119, the present study was designed to elucidate the distribution of GPR119 gene expressionin humans. GPR119 mRNA expression was measured in fresh human tissues collected at surgery. GPR119 mRNA was detected most abundantly in the pancreas, followed by the duodenum, stomach, jejunum, ileum and colon. GPR119 mRNA in pancreatic islets was enriched more than 10-fold as compared with adjacent pancreatic tissue and its level was comparable to that of GPR40 mRNA, which is highly expressed in human pancreatic islets. GPR119 mRNA was also abundant in two cases of insulinoma and two cases of glucagonoma, but was undetectable in a pancreatic acinar cell tumor. The present study demonstrates that GPR119 mRNA is abundantly expressed in human pancreatic islets and gastrointestinal tract, as well as in human insulinomas and glucagonomas, indicating islet-gastrointestinal distribution of GPR119 and its expression in pancreatic beta and alpha cells, as well as the possible involvement in islet functions in humans.
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