2013 Fiscal Year Final Research Report
A novel GDP-bound-Rab27a-interacting protein which regulates endocytosis of insulin secretory membranes
| Project/Area Number |
23791039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | IQGAP1 / Rab27a / エンドサイトーシス / 膵B細胞 / インスリン / 糖尿病 / Gタンパク質 / メンブレントラフィック |
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| Research Abstract |
Intracellular trafficking is a fundamental cellular process that is required for communication between organelles. Recruitment of specific molecules to specific membrane sites is essential for this trafficking. Rab27a, a member of the Rab GTPase family, has been considered to act as a molecular switch for exocytosis in insulin secretion, cycling between an active GTP-bound and an inactive GDP-bound state. I previously showed that GDP-bound Rab27a is not an inactive form but is in fact an active form that regulates endocytosis through the GDP-dependent effector coronin3. In the present study, I identified IQGAP1, an effector of GTP-bound Cdc42, as another GDP-dependent effector of Rab27a. My results indicate that activation of Cdc42 in response to the insulin secretagogue glucose recruits endocytic machinery, including GDP-bound Rab27a and coronin3, to IQGAP1 at the cell periphery and regulates endocytosis at this membrane site.
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Research Products
(20 results)
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[Journal Article] Activated Cdc42-bound-IQGAP1 determines the cellular endocytic site2013
Author(s)
Kimura T, Yamaoka M, Taniguchi S, Okamoto M, Takei M, Ando T, Iwamatsu A, Watanabe T, Kaibuchi K, Ishizaki T, Niki I
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Journal Title
Mol. Cell. Biol
Volume: 33
Pages: 4834-43
DOI
Peer Reviewed
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[Journal Article] Distribution of hydrogen sulfide (H2S)-producing enzymes and the roles of the H2S donor sodium hydrosulfide in diabetic nephropathy2013
Author(s)
Yamamoto J, Sato W, Kosugi T, Yamamoto T, Kimura T, Taniguchi S, Kojima H, Maruyama S, Imai E, Matsuo S, Yuzawa Y, Niki I
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Journal Title
Clin Exp Nephrol
Volume: 17
Pages: 32-40
DOI
Peer Reviewed
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