2012 Fiscal Year Final Research Report
Elucidation of the localization of hematopoietic stem cells and their niche using a novel cell surface marker, Plxdc2
| Project/Area Number |
23791083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 移植・再生医療 / 発生・分化 / 造血幹細胞 / ニッチ / Plxdc2 |
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| Research Abstract |
We performed a stringent comparative gene expression profiling analysis to find genes preferentially expressed in the HSC population, and identified a total of 63 genes that are highly expressed in HSC among various hematopoietic cell population.In order to find HSC-specific marker we focused on genes encoding cell surface protein, and found that plexin domain containing 2 (Plxdc2) is highly expressed in CD34－c-Kit+Sca-1+Lineage-(CD34－KSL) HSC population using Plxdc2::GFP knock-in mice. Only 0.2% of whole bone marrow cells were Plxdc2+. Furthermore, Plxdc2+CD150+fraction represented only 0.01%±0.002% in whole bone marrow, suggesting that the simple combination of Plxdc2 and CD150 significantly increased HSC purity.
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[Presentation] Concurrent development of invasive cryptococcosis and mature B-cell neoplasm in an HIV-negative young adult2012
Author(s)
Itamura H, Hisatomi T, Yoshimura M, Yokoo M, Ide M, Kubota Y, Fukushima N, Yoshihara M, Nagata M, Ichinohe T, Ohshima K, Aoki Y, Sueoka E, Kimura S
Organizer
第74回日本血液学会学術集会
Place of Presentation
京都
Year and Date
20121019-21
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[Presentation] Antiproliferative Effect of 2-Hydroxypropyl-ss-Cyclodextrin (HP-ss-CyD) Against Chronic Myeloid Leukemia In Vitro and In Vivo2012
Author(s)
Yokoo M, Kubota Y, Mochinaga S, Maeda A, Ichinohe T, Fujito H, Higashi T, Motoyama K, Arima H, Irie T, Kimura S
Organizer
The 54th Annual Meeting of the American Society of Hematology
Place of Presentation
Atlanta, USA
Year and Date
20121008-11
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[Presentation] The role of hypoxia inducible factors on maintenance of hematopoietic stem cells and leukemogenesis2012
Author(s)
Sueoka E, Ide M, Sueoka-Aragane N, Sato A, Nakamura H, Sotomaru Y, Taya C, Yonekawa H, Nakachi K, Kubota Y, Kimura S, Tanimoto K
Organizer
2012 Tumor Microenvironment GCRC International Symposium.
Place of Presentation
Seoul
Year and Date
20120823-24
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[Presentation] Successful reduced-intensity umbilical cord blood trans plantation for adult refractory hemophagocytic syndrome withanti-HLA antigen.2012
Author(s)
Itamura H,Fukushima N,Kondo S,Urata C,Yoshimura M,Yokoo M,Ide M,Hisatomi T, Kubota Y, Sueoka E, Ichinohe T, Kimura S.
Organizer
第34回日本造血細胞移植学会学術集会
Place of Presentation
大阪
Year and Date
20120224-25
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[Presentation] Relation between CNS involvement and involved extranodal site at presentation in DLBL2011
Author(s)
Itamura H, Fukushima N, Urata C, Yoshimura M, Yokoo M, Ide M, Hisatomi T, Kubota Y, Ichinohe T, Sueoka E, Kimura S.
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋
Year and Date
20111014-16
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[Presentation] Hemostatic abnormality in patients with adult T-cell leukemia-lymphoma2011
Author(s)
Kubota Y, Sano M, Kubota M, Itamura H, Urata C, Yoshimura M, Hisatomi T, Fukushima N, Sueoka E, Ichinohe T, Kimura S
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋
Year and Date
20111014-16
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[Presentation] 当院における濾胞型リンパ腫82例の臨床成績の検討2011
Author(s)
北村浩晃, 吉村麻里子, 福島伯泰, 板村英和, 浦田千紗子, 横尾眞子, 出勝, 久富崇, 久保田寧, 一戸辰夫, 末岡榮三朗, 木村晋也
Organizer
第51回日本リンパ網内系学会総会
Place of Presentation
福岡
Year and Date
2011-07-01
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