2013 Fiscal Year Final Research Report
Interaction of Aspergillus biofilm and host immune cells.
| Project/Area Number |
23791138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | アスペルギルス / バイオフィルム / 免疫応答 / αーグルカン |
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| Research Abstract |
Biofilm formation of fungus has been thought to play an important role in chronic pulmonary aspergillosis. In this study, I analyzed the structure of Aspergillus BF, and the interaction of BF with host immune cells. In vitro Aspergillus fumigatus biofilm model, alfa-glucan was present on the surface of fungal cells. Next, when the fungal cells were treated with alfa-glucanase, aggregation of cells was strongly inhibited. When the fungal cells were co-incubated with alfa-glucanase, cytokines production, such as IL-8 and TNF-alfa, from host immune cells (THP-1 cells) was increased. These results suggest that alfa-glucan plays an important role in biofilm formation of Aspergillus fumigatus, and alfa-glucanase might be utilized for inhibition of biofilms.
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