Developing a novel therapy with bone marrow mononuclear cells for perinatal brain injuries

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791220
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Nagoya University
• Principal Investigator: SATO YOSHIAKI 名古屋大学, 医学部附属病院, 講師 (30435862)
• Project Period (FY): 2011 – 2013
• Keywords: 周産期脳障害 / 骨髄単核球

Research Abstract

It is urgent to develop novel therapies for infants with perinatal hypoxic-ischemic encephalopathy (HIE). In the present project, we have performed basic experiments to develop a novel therapy with bone marrow mononuclear cells (BM-MNC), which is most ideal source of stem cells for the treatment against perinatal brain injuries. We evaluated the effect of administration of BM-MNC on perinatal HIE rat. We injected BM-MNC intravenously after hypoxic-ischemic insult. The injected BM-MNC was detected in the lung less than when we administered bone marrow mesenchymal stem cells. BM-MNC administration exerted anti-apoptotic effect, and tended to ameliorate learning impairment induced by HIE.

Research Products

• [Journal Article] Paradoxical downward seizure pattern on amplitude-integrated electroencephalogram (2014)
  • Author(s): Ito M, Kidokoro H, Sugiyama Y, Sato Y, Natsume J, Watanabe K, Hayakawa M
  • Journal Title: J Perinat Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Effects of intravenous administration of umbilical cord blood CD34 cells in a mouse model of neonatal stroke (2014)
  • Author(s): Tsuji M, Taguchi A, Ohshima M, Kasahara Y, Sato Y, Tsuda H, Otani K, Yamahara K, Ihara M, Harada-Shiba M, Ikeda T, Matsuyama T
  • Journal Title: Neuroscience Volume: 263C Pages: 148-58
  • DOI: 10.1016/j.neuroscience.2014.01.018
  • Peer Reviewed
• [Journal Article] Effect of placenta previa on neonatal respiratory disorders and amniotic lamellar body counts at 36-38weeks of gestation (2014)
  • Author(s): Tsuda H, Kotani T, Sumigama S, Mano Y, Hua L, Hayakawa H, Hayakawa M, Sato Y, Kikkawa F
  • Journal Title: Early Hum Dev Volume: 90(1) Pages: 51-4
  • DOI: 10.1016/j.earlhumdev.2013.10.005
  • Peer Reviewed
• [Journal Article] Amniotic lamellar body count and congenital diaphragmatic hernia in humans and in a rat model (2013)
  • Author(s): Watanabe Y, Tsuda H, Kotani T, Sumigama S, Mano Y, Hayakawa M, Sato Y, Kikkawa F
  • Journal Title: Pediatr Res Volume: 73(3) Pages: 344-8
  • DOI: 10.1038/pr.2012.187
  • Peer Reviewed
• [Journal Article] Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer (2013)
  • Author(s): Sato Y, Shinjyo N, Sato M, Osato K, Zhu C, Pekna M, Kuhn HG, Blomgren K
  • Journal Title: Cell Death Dis Volume: 4 Pages: e591
  • DOI: 10.1038/cddis.2013.92
  • Peer Reviewed
• [Journal Article] The diameter of the inferior vena cava provides a noninvasive way of calculating central venous pressure in neonates (2013)
  • Author(s): Sato Y, Kawataki M, Hirakawa A, Toyoshima K, Kato T, Itani Y, Hayakawa M
  • Journal Title: Acta Paediatr Volume: 102(6) Pages: e241-6
  • DOI: 10.1111/apa.12247
  • Peer Reviewed
• [Journal Article] Dexamethasone administration to the neonatal rat results in neurological dysfunction at the juvenile stage even at low doses (2013)
  • Author(s): Ichinohashi Y, Sato Y, Saito A, Ito M, Watanabe K, Hayakawa M, Nakanishi K, Wakatsuki A, Oohira
  • Journal Title: Early Hum Dev Volume: 89(5) Pages: 283-8
  • DOI: 10.1016/j.earlhumdev.2012.10.007
  • Peer Reviewed
• [Journal Article] Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice (2013)
  • Author(s): Ibi D, Nagai T, Nakajima A, Mizoguchi H, Kawase T, Tsuboi D, Kano S, Sato Y, Hayakawa M, Lange UC, Adams DJ, Surani MA, Satoh T, Sawa A, Kaibuchi K, Nabeshima T, Yamada K
  • Journal Title: Glia Volume: 61(5) Pages: 679-93
  • DOI: 10.1002/glia.22461
  • Peer Reviewed
• [Journal Article] A highly-sulfated chondroitin sulfate, CS-E, adsorbs specifically to neurons with nuclear condensation (2012)
  • Author(s): Nakanishi K, Ito M, Sato Y, Oohira
  • Journal Title: Neurosci Res Volume: 74(3-4) Pages: 223-7
  • DOI: 10.1016/j.neures.2012.08.009
  • Peer Reviewed
• [Journal Article] Congenital pancreatoblastoma associated with beta-catenin mutation (2012)
  • Author(s): Ismael O, Shimada A, Hama A, Takahashi Y, Sato Y, Hayakawa M, Tsuchiya H, Tainaka T, Ono Y, Kaneko K, Ando H, Sato K, Kojima S
  • Journal Title: Pediatric blood & cancer Volume: 58(5) Pages: 827
  • DOI: 10.1002/pbc.23337
  • Peer Reviewed
• [Journal Article] 中枢神経疾患に対する幹細胞療法 (2011)
  • Author(s): 佐藤義朗,中西圭子,服部哲夫,一ノ橋祐子, Blomgren K,大平敦彦,早川昌弘
  • Journal Title: 脳と発達 Volume: 43(3) Pages: 33-7
• [Journal Article] 周産期脳障害に対する幹細胞療法 (2011)
  • Author(s): 佐藤義朗
  • Journal Title: 周産期医学 Volume: 11 Pages: 1531-6
• [Journal Article] Carbohydrate and energy metabolism in the brain of rats with thromboxane A2-induced fetal growth restriction (2011)
  • Author(s): Hayakawa M, Sato Y, Hattori T, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Ito M, Ieda K, Kojima S
  • Journal Title: Pediatr Res Volume: 70(1) Pages: 21-4
  • DOI: 10.1203/PDR.0b013e31821b9d7c
  • Peer Reviewed
• [Presentation] 新生児低酸素性虚血性脳症モデルラットにおける各種細胞別の投与後体内動態の検討 (2014)
  • Author(s): 杉山裕一朗, 佐藤義朗, 近藤大貴. 鈴木俊彦, 山本徳則, 鈴木哲, 中西圭子, 辻雅弘, 早川昌弘
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都市国立京都国際会館
  • Year and Date: 20140304-06
• [Presentation] Administration of human umbilical cord blood cells reduce hypoxic-ischemic brain injury in the neonatal rat (2013)
  • Author(s): T. Hattori, Y. Sato, T. Kondo, Y. Ichinohashi, S. Suzuki, M. Yamamoto, T. Kotani, H. Hirata, K. Nakanishi, M. Hayakawa
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, USA
  • Year and Date: 20131109-13
• [Presentation] 新生児低酸素性虚血性脳症モデルラットを用いた移植細胞体内動態の検討 (2013)
  • Author(s): 近藤大貴, 佐藤義朗, 杉山裕一朗, 見松はるか, 伊藤美春, 齊藤明子, 服部哲夫, 藤巻英彦, 一ノ橋祐子, 中西圭子, 早川昌弘
  • Organizer: 第49回日本周産期・新生児医学会学術集会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 20130714-16
• [Presentation] 新生児脳障害モデルラットに対するラット臍帯血幹細胞投与の検討 (2013)
  • Author(s): 中西圭子、伊藤美春、佐藤義朗、東雄二郎
  • Organizer: 第49回日本周産期・新生児医学会学術集会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 20130714-16
• [Presentation] Stem cell therapies for perinatal brain injuries (2013)
  • Author(s): Yoshiaki Sato
  • Organizer: XI World Congress of Perinatal Medicine
  • Place of Presentation: モスクワ、ロシア
  • Year and Date: 20130619-22
  • Invited
• [Presentation] 新生児低酸素性虚血性脳症に対する幹細胞療法 (2013)
  • Author(s): 佐藤義朗
  • Organizer: 第39回日本小児神経学会東海地方会
  • Place of Presentation: 名古屋市名古屋大学医学部特別講演
  • Year and Date: 2013-07-20
• [Presentation] 新生児低酸素性虚血性脳症に対するヒト臍帯血由来単核球を用いた幹細胞療法 (2012)
  • Author(s): 服部哲夫、佐藤義朗、杉山裕一朗、津田弘之、伊藤美春、近藤大貴、齊藤明子、藤巻英彦、一ノ橋祐子、中西圭子、早川昌弘
  • Organizer: 第57回日本未熟児新生児学会学術集会
  • Place of Presentation: 熊本市ホテル日航熊本
  • Year and Date: 20121125-27
• [Presentation] 周産期低酸素性虚血性脳症に対する幹細胞療法 (2012)
  • Author(s): 佐藤義朗
  • Organizer: 12th Conference for BioSignal and Medicine
  • Place of Presentation: 志摩市ヴィラ伊勢志摩
  • Year and Date: 20120831-0902
  • Invited
• [Presentation] 新生児低酸素性虚血性脳症に対するヒト臍帯血由来単核球を用いた幹細胞療法 (2012)
  • Author(s): 服部哲夫、佐藤義朗、杉山裕一朗、伊藤美春、近藤大貴、齊藤明子、藤巻英彦、一ノ橋祐子、山本美知郎、小谷友美、平田仁、中西圭子、早川昌弘
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜パシフィコ横浜
  • Year and Date: 20120321-23
• [Patent(Industrial Property Rights)] 低酸素性虚血性脳症の予防又は治療剤及びその利用 (2014)
  • Inventor(s): 佐藤義朗 齊藤明子
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-22411
  • Filing Date: 2014-02-07
  • Overseas
• [Patent(Industrial Property Rights)] 脳損傷抑制剤 (2013)
  • Inventor(s): 大平敦彦、佐藤義朗、中西圭子、前田 浩
  • Industrial Property Rights Holder: 愛知県、生化学工業株式会社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特許第5279491号
  • Acquisition Date: 2013-05-31
  • Overseas