Immunotherapy utilizing dendritic cells derived from iPS cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kumamoto University
• Principal Investigator: FUKUSHIMA Satoshi 熊本大学, 医学部附属病院, 講師 (50398210)
• Project Period (FY): 2011 – 2012
• Keywords: 悪性黒色腫 / 免疫療法 / iPS 細胞

Research Abstract

We establish a streamlined process to generate the dendritic cells from mouse iPS cells and ES cells. To enhance the anti-tumor effect, we generated a ES cell clone expressing IL-15. Furthermore, we generated the macrophages derived from human iPS cells as an effector cells attacking tumor . The iPS cell derived macrophages itself did not show any anti-tumor effects on human melanoma grafted SCID mouse model. However ,genetically modified cytokine producing iPS macrophages show a potent anti tumor effects.

Research Products

• [Journal Article] The rs2910164 G>C polymorphism inmicroRNA-146a is associated with theincidence of malignant melanoma (2013)
  • Author(s): Yamashita J, Iwakiri T, Fukushima S,Jinnin M, Miyashita A, Hamasaki T,Makino T, Aoi J, Masuguchi S, Inoue Y,Ihn H
  • Journal Title: Melanoma Res Volume: 23巻 Pages: 13-20
  • DOI: DOI:10.1097/CMR.0b013e32835c5b30
  • Peer Reviewed
• [Journal Article] miR-150 down-regulation contributesto the constitutive type I collagenoverexpression in scleroderma dermalfibroblasts via the induction ofintegrin β3 (2013)
  • Author(s): Honda N, Jinnin M,Kira-Etoh T, Makino K, Kajihara I,Makino T, Fukushima S, Inoue Y,Okamoto Y, Hasegawa M, Fujimoto M, IhnH
  • Journal Title: Am J Pathol Volume: 182巻 Pages: 206-16
  • DOI: DOI:10.1016/j.ajpath.2012.09.023
  • Peer Reviewed
• [Journal Article] Serum miR-21 levels in patients withdermatomyositis (2013)
  • Author(s): Shimada S, Jinnin M,Ogata A, Makino T, Kajihara I, MakinoK, Honda N, Nakayama W, Inoue K,Fukushima S, Ihn H
  • Journal Title: Clin Exp Rheumatol Volume: 31巻 Pages: 161-2
  • Peer Reviewed
• [Journal Article] Intravenous immunoglobulin treatmentrecovers the down-regulated levels ofTh1 cytokines in the sera and skin ofscleroderma patients (2013)
  • Author(s): Kudo H, JinninM, Yamane K, Makino T, Kajihara I,Makino K, Honda N, Nakayama W,Fukushima S, Ihn H
  • Journal Title: J Dermatol Sci Volume: 69巻 Pages: 77-80
  • DOI: DOI:10.1016/j.jdermsci.2012.09.010
  • Peer Reviewed
• [Journal Article] Decreased miR-7 Expression in theSkin and Sera of Patients withDermatomyositis (2013)
  • Author(s): Oshikawa Y, Jinnin M,Makino T, Kajihara I, Makino K, HondaN, Nakayama W, Inoue K, Fukushima S,Ihn H
  • Journal Title: Acta Derm Venereol Volume: 93巻 Pages: 273-6
  • DOI: DOI:10.2340/00015555-1459
  • Peer Reviewed
• [Journal Article] microRNA-7 down-regulation mediatesexcessive collagen expression inlocalized scleroderma (2013)
  • Author(s): Etoh M, JinninM, Makino K, Yamane K, Nakayama W, AoiJ, Honda N, Kajihara I, Makino T,Fukushima S, Ihn H
  • Journal Title: Arch Dermatol Res Volume: 305巻 Pages: 9-15
  • DOI: DOI:10.1007/s00403-012-1287-4
  • Peer Reviewed
• [Journal Article] Discoidin domain receptor 2-microRNA196a-mediated negative feedbackagainst excess type I collagenexpression is impaired in sclerodermadermal fibroblasts (2013)
  • Author(s): Makino K, JinninM, Aoi J, Hirano A, Kajihara I, MakinoT, Sakai K, Fukushima S, Inoue Y, IhnH
  • Journal Title: J Invest Dermatol Volume: 33巻 Pages: 110-9
  • DOI: DOI:10.1038/jid.2012.252
  • Peer Reviewed
• [Journal Article] Down-regulation of miR-124/-214 incutaneous squamous cell carcinomamediates abnormal cell proliferationvia the induction of ERK (2013)
  • Author(s): Yamane K,Jinnin M, Etoh T, Kobayashi Y,Shimozono N, Fukushima S, Masuguchi S,Maruo K, Inoue Y, Ishihara T, Aoi J,Oike Y, Ihn H
  • Journal Title: J Mol Med (Berl) Volume: 91巻 Pages: 69-81
  • DOI: DOI:10.1007/s00109-012-0935-7
  • Peer Reviewed
• [Journal Article] Scleroderma dermal fibroblastsoverexpress vascular endothelialgrowth factor due to autocrinetransforming growth factor βsignaling (2013)
  • Author(s): Kajihara I, Jinnin M,Honda N, Makino K, Makino T, MasuguchiS, Sakai K, Fukushima S, Inoue Y, IhnH
  • Journal Title: Mod Rheumatol Volume: 23巻 Pages: 516-24
  • DOI: DOI:10.1007/s10165-012-0698-6
  • Peer Reviewed
• [Journal Article] Kinesin family member 20A is a novelmelanoma-associated antigen (2012)
  • Author(s): Yamashita J, Fukushima S, Jinnin M,Honda N, Makino K, Sakai K, MasuguchiS, Inoue Y, Ihn H
  • Journal Title: Acta Derm Venereol Volume: 92巻 Pages: 593-7
  • DOI: DOI:10.2340/00015555-1416
  • Peer Reviewed
• [Journal Article] Photosensitivity and acute liverinsufficiency in late-onseterythropoietic protoporphyria with achromosome 18q abnormality (2012)
  • Author(s): OshikawaY, Fukushima S, Miyake T, Kawaguchi T,Motomura K, Nakashima Y, Nakamura K,Jinnin M, Ihn H
  • Journal Title: Case Rep Dermatol Volume: 4巻 Pages: 144-9
  • DOI: DOI:10.1159/000341111
  • Peer Reviewed
• [Journal Article] Angiogenin expression in the sera andskin of patients with rheumaticdiseases (2012)
  • Author(s): Kuwahara A, Jinnin M, MakinoT, Kajihara I, Makino K, Honda N,Nakayama W, Inoue K, Fukushima S, IhnH
  • Journal Title: Biosci Trends Volume: 5巻 Pages: 229-33
  • URL: http://www.biosciencetrends.com/getabstract.php?id=590
  • Peer Reviewed
• [Journal Article] Case of blastic plasmacytoiddendritic cell neoplasm (2012)
  • Author(s): Aoi J, OgataA, Makino T, Sakai K, Masuguchi S,Fukushima S, Jinnin M, Inoue Y, Ihn H
  • Journal Title: J Dermatol Volume: 39巻 Pages: 1066-7
  • DOI: DOI:10.1111/j.1346-8138.2012.01656.x
  • Peer Reviewed
• [Journal Article] microRNA-92a expression in the seraand dermal fibroblasts increases inpatients with scleroderma (2012)
  • Author(s): Sing T,Jinnin M, Yamane K, Honda N, Makino K,Kajihara I, Makino T, Sakai K,Masuguchi S, Fukushima S, IhnH
  • Journal Title: Rheumatology (Oxford) Volume: 51巻 Pages: 1550-6
  • DOI: DOI:10.1093/rheumatology/kes120
  • Peer Reviewed
• [Journal Article] Serum angiopoietin-like protein 3concentrations in rheumatic diseases (2012)
  • Author(s): Hayashi Y, Jinnin M, Makino T,Kajihara I, Makino K, Honda N,Nakayama W, Inoue K, Fukushima S, IhnH
  • Journal Title: Eur J Dermatol Volume: 22巻 Pages: 500-4
  • DOI: DOI:10.1684/ejd.2012.1774
  • Peer Reviewed
• [Journal Article] CD163 expression is increased in theinvolved skin and sera of patientswith systemic lupus erythematosus (2012)
  • Author(s): Nakayama W, Jinnin M, Makino K,Kajihara I, Makino T, Fukushima S,Sakai K, Inoue Y, Ihn H
  • Journal Title: Eur J Dermatol Volume: 22巻 Pages: 512-7
  • DOI: DOI:10.1684/ejd.2012.1756
  • Peer Reviewed
• [Journal Article] Impaired IL-17 signaling pathwaycontributes to the increased collagenexpression in sclerodermafibroblasts (2012)
  • Author(s): Nakashima T, Jinnin M,Yamane K, Honda N, Kajihara I, MakinoT, Masuguchi S, Fukushima S, OkamotoY, Hasegawa M, Fujimoto M, Ihn H
  • Journal Title: J Immunol Volume: 188巻 Pages: 3573-83
  • DOI: DOI:10.4049/jimmunol.1100591
  • Peer Reviewed
• [Journal Article] TGF-β-mediated downregulation ofmicroRNA-196a contributes to theconstitutive upregulated type Icollagen expression in sclerodermadermal fibroblasts (2012)
  • Author(s): Honda N, Jinnin M,Kajihara I, Makino T, Makino K,Masuguchi S, Fukushima S, Okamoto Y,Hasegawa M, Fujimoto M, Ihn H
  • Journal Title: J Immunol Volume: 188巻 Pages: 3323-31
  • DOI: DOI:10.4049/jimmunol.1100876
  • Peer Reviewed
• [Journal Article] The role of angiopoietin-like protein2 in pathogenesis of dermatomyositis (2012)
  • Author(s): Ogata A, Endo M, Aoi J, Takahashi O,Kadomatsu T, Miyata K, Tian Z, JinninM, Fukushima S, Ihn H, Oike Y
  • Journal Title: BiochemBiophys Res Commun Volume: 418巻 Pages: 494-9
  • DOI: DOI:10.1016/j.bbrc.2012.01.052
  • Peer Reviewed
• [Journal Article] Increased accumulation ofextracellular thrombospondin-2 dueto low degradation activitystimulates type I collagen expressionin scleroderma fibroblasts (2012)
  • Author(s): KajiharaI, Jinnin M, Yamane K, Makino T, Honda N, Igata T, Masuguchi S, Fukushima S,Okamoto Y, Hasegawa M, Fujimoto M, IhnH
  • Journal Title: Am J Pathol Volume: 180巻 Pages: 703-14
  • DOI: DOI:10.1016/j.ajpath.2011.10.030
  • Peer Reviewed
• [Journal Article] Increased serum levels of miR-1266 inpatients with psoriasis vulgaris (2012)
  • Author(s): Ichihara A, Jinnin M, Oyama R, YamaneK, Fujisawa A, Sakai K, Masuguchi S,Fukushima S, Maruo K, Ihn H
  • Journal Title: Eur J Dermatol Volume: 22巻 Pages: 68-71
  • DOI: DOI:10.1684/ejd.2011.1600
  • Peer Reviewed
• [Journal Article] Evaluation of usefulness of 3D viewsfor clinical photography (2011)
  • Author(s): Jinnin M,Fukushima S, Masuguchi S, Tanaka H,Kawashita Y, Ishihara T, Ihn H
  • Journal Title: Biosci Trends Volume: 5巻 Pages: 211-6
  • URL: http://www.biosciencetrends.com/getabstract.php?id=463
  • Peer Reviewed
• [Journal Article] 多能性幹細胞から誘導した樹状細胞によるがん免疫療法 (2011)
  • Author(s): 福島聡、尹浩信、西村泰治、千住覚
  • Journal Title: 日本臨床免疫学会会誌 Volume: 34巻 Pages: 113-120
• [Journal Article] Adiponectin expression is decreasedin the involved skin and sera ofdiffuse cutaneous sclerodermapatients (2011)
  • Author(s): Arakawa H, Jinnin M,Muchemwa FC, Makino T, Kajihara I,Makino K, Honda N, Sakai K, FukushimaS, Ihn H
  • Journal Title: Exp Dermatol Volume: 20巻 Pages: 764-6
  • DOI: DOI:10.1111/j.1600-0625.2011.01310.x
  • Peer Reviewed
• [Journal Article] Immunohistochemicalcharacterization of the cellularinfiltrate in discoid lupuserythematosus (2011)
  • Author(s): Xie Y, Jinnin M, ZhangX, Wakasugi S, Makino T, Inoue Y,Fukushima S, Masuguchi S, Sakai K, IhnH
  • Journal Title: Biosci Trends Volume: 5巻 Pages: 83-8
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21572252
  • Peer Reviewed
• [Journal Article] Immunotherapy with pluripotent stem cell-derived dendritic cells (2011)
  • Author(s): Senju S,Matsunaga Y, Fukushima S, Hirata S,Motomura Y, Fukuma D, Matsuyoshi H,Nishimura Y
  • Journal Title: Semin Immunopathol Volume: 33巻 Pages: 603-12
  • DOI: DOI:10.1007/s00281-011-0263-y
• [Presentation] Decreased adiponectinlevels in the patients with diffusecutaneous systemic sclerosis (2012)
  • Author(s): Fukushima S
  • Organizer: 37thAnnual Meeting of the JSID
  • Place of Presentation: ロワジールホテル沖縄、那覇
  • Year and Date: 20121207-09
• [Presentation] The rs2910164 G>Cpolymorphism in microRNA-146a isassociated with the incidence ofmalignant melanoma (2012)
  • Author(s): Fukushima S
  • Organizer: 42nd Annual ESDRMeeting
  • Place of Presentation: Venice LidoCongress Centre, Venice, Italy
  • Year and Date: 20120919-22
• [Presentation] メラノーマに対する化学療法 upto date (2012)
  • Author(s): 福島聡
  • Organizer: 第28回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 京王プラザホテル札幌、札幌
  • Year and Date: 20120629-30
• [Presentation] メラノーマの免疫療法とバイオマーカー (2012)
  • Author(s): 福島聡
  • Organizer: 第111回日本皮膚科学会総会
  • Place of Presentation: 国立京都国際会館、京都
  • Year and Date: 20120601-03
• [Presentation] ロクロニウムによるアナフィラキシーの1例 (2012)
  • Author(s): 福島聡
  • Organizer: 第24回日本アレルギー学会春季臨床大会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 20120512-13
• [Presentation] A novel tumor marker ofmalignant melanoma, the circulatingmicroRNA-221 (2011)
  • Author(s): Fukushima S
  • Organizer: The 36th Annual meetingof the JSID
  • Place of Presentation: 国立京都国際会館, Kyoto
  • Year and Date: 20111209-11
• [Presentation] 膠原病と類縁疾患 強皮症関連病態における血清 microRNA-29a (2011)
  • Author(s): 福島聡
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: グランドプリンスホテル新高輪 国際館パミール、東京
  • Year and Date: 20111110-12
• [Presentation] A new tumor marker ofmalignant melanoma, the circulatingmicroRNA-221 (2011)
  • Author(s): Fukushima S
  • Organizer: 41st Annual ESDRmeeting
  • Place of Presentation: Barcelona,Spain
  • Year and Date: 20110907-10
• [Presentation] 悪性黒色腫の新規血清マーカーとしての microRNA-221 (2011)
  • Author(s): 福島聡
  • Organizer: 第27回日本皮膚悪性腫瘍学会
  • Place of Presentation: 京王プラザホテル東京、東京
  • Year and Date: 20110603-04
• [Presentation] Increased serum levels ofSPARC in patients with localizedscleroderma (2011)
  • Author(s): Fukushima S
  • Organizer: 2ndWorld Congress ofDermatology
  • Place of Presentation: Seoul,Korea
  • Year and Date: 20110524-29
• [Presentation] 留置針アレルギーが疑われた機械性蕁麻疹の1例 (2011)
  • Author(s): 福島聡
  • Organizer: 第23回日本アレルギー学会春季臨床大会
  • Place of Presentation: 幕張メッセ、東京
  • Year and Date: 20110514-15
• [Book] 【治りにくい皮膚病変 治療の実際】 悪性黒色腫 (2013)
  • Author(s): 福島聡
  • Total Pages: 57-63
  • Publisher: Derma