Elucidation of division control and aging mechanism in single mesenchymal stem cell.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Keio University
• Principal Investigator: MABUCHI Yo 慶應義塾大学, 医学部, 共同研究員 (50424172)
• Project Period (FY): 2011 – 2012
• Keywords: 放射線治療生物学 / 間葉系幹細胞

Research Abstract

Mesenchymal stem cells (MSCs), conventionally isolated based on their adherence to plastic are heterogeneous and have poor growth and differentiation, limiting the ability to investigate their intrinsic characteristics. We elucidate the division control a

Research Products

• [Journal Article] Mesenchymal Stem Cells Regulate Epithelial-to-Mesenchymal Transition and Tumor Progression of Pancreatic Cancer Cells (2013)
  • Author(s): Kabashima-Niibe A, Higuchi H, Takaishi H, Masugi Y, Matsuzaki Y, Mabuchi Y, Funakoshi S, Adachi M, Hamamoto Y, Kawachi S, Aiura K, Kitagawa Y, Sakamono M, Hibi T
  • Journal Title: Cancer Sci Volume: 104(2) Pages: 157-164
  • DOI: doi:10.1111/cas.12059
  • Peer Reviewed
• [Journal Article] Primary evaluation of induced pluripotent stem cells using flow cytometry (2013)
  • Author(s): Araki D, Kawamura Y, Niibe K, Suzuki S, Morikawa S, Mabuchi Y, Nakagawa T, Okano H and Matsuzaki Y.
  • Journal Title: Inflammation and Regeneration Volume: 33(1) Pages: 3-12
  • Peer Reviewed
• [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α (2012)
  • Author(s): Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y.(These authors contributed equally to this work.)
  • Journal Title: Nat Protoc Volume: 7(12) Pages: 2103-2111
  • DOI: doi:10.1038/nprot.2012.125
  • Peer Reviewed
• [Journal Article] Discovering the true identity and function of mesenchymal stem cells (2012)
  • Author(s): Mabuchi Y, Houlihan DD, Okano H, Matsuzaki Y.
  • Journal Title: Inflammation and Regeneration Volume: 32(4) Pages: 146-151
  • Peer Reviewed
• [Journal Article] Transplantation of Side Population Cells Restores the Function of Damaged Exocrine Glands Through Clusterin. (2012)
  • Author(s): Mishima K, Inoue H, Nishiyama T, Mabuchi Y, Amano Y, Ide F, Matsui M, Yamada H, Yamamoto G, Tanaka J, Yasuhara R, Sakurai T, Lee MC, Chiba K, Sumimoto H, Kawakami Y, Matsuzaki Y, Tsubota K, Saito I.
  • Journal Title: Stem Cells. Volume: 30(9) Pages: 1925-1937
  • DOI: doi:10.1002/stem.1173
  • Peer Reviewed
• [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathways (2012)
  • Author(s): Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
  • Journal Title: PLoS One Volume: 7(3) Pages: e33431
  • DOI: doi:10.1371/journal.pone.0033431
  • Peer Reviewed
• [Journal Article] Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition. (2011)
  • Author(s): Renault-Mihara F, Katoh H, Ikegami T, Iwanami A, Mukaino M, Yasuda A, Nori S, Mabuchi Y, Tada H, Shibata S, Saito K, Matsushita M, Kaibuchi K, Okada S, Toyama Y, Nakamura M, Okano H
  • Journal Title: EMBO Mol Med Volume: 3(11) Pages: 682-696
  • DOI: doi:10.1002/emmm.201100179.
  • Peer Reviewed
• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Niibe K, Kawamura Y, Araki D, Morikawa S, Miura K, Suzuki S, Shimmura S, Sunabori T, Mabuchi Y, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: PLoS One Volume: 6(3) Pages: e17610
  • DOI: doi:10.1371/journal.pone.0017610
  • Peer Reviewed
• [Presentation] LNGFR and THY-1 based prospective isolation of human mesenchymal stem cells reveals functionally distinct subpopulations. (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Sato Y, Francois R. M., Diarmaid D. H, Okano H & Matsuzaki Y.
  • Organizer: International Society for Stem Cell Research (ISSCR), 10th Annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 20120613-16
• [Presentation] Identification of selective markers for highly purified human mesenchymal stem/stromal cells (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Francois R. M., Diarmaid D. H, Okano H & Matsuzaki Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] Identification of selective markers for highly purified human mesenchymal stem/stromal cells. (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Francois R. M., Diarmaid D. H, Akazawa C, Okano H & Matsuzaki Y
  • Organizer: G-COE Final Symposium
  • Place of Presentation: 東京
  • Year and Date: 2012-12-04
• [Presentation] ヒト間葉系幹細胞の予期的分離と機能的解析 (2012)
  • Author(s): 馬渕洋、岡野栄之、松崎有未
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-07-05
• [Presentation] Single cell isolation elucidates heterogeneity within the multi-potent stem cell compartment. (2011)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Okano H, Matsuzaki Y
  • Organizer: International Society for Stem Cell Research (ISSCR), 9th Annual Meeting
  • Place of Presentation: Canada
  • Year and Date: 20110615-18
• [Remarks]
  • URL: http://www.okano-lab.com/publication