2012 Fiscal Year Final Research Report

Identification of stromal cells that have suppressive role againstpancreatic cancer cells and development of new anticancer therapy

Research Project

Project/Area Number	23791544
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Digestive surgery
Research Institution	Kyushu University
Principal Investigator	MORIYAMA Taiki 九州大学, 医学研究院, 共同研究員 (70586859)
Project Period (FY)	2011 – 2012
Keywords	膵癌 / 膵星細胞 / CD271
Research Abstract	The aim of the present study was to identify subpopulation of stromal cells that suppresspancreatic cancer cells. We analyzed surface marker of pancreatic stellate cells(PSCs) derived from human pancreatic ductal adenocarcinoma(PDAC), and characterized CD271+ PSCs.Quantitative RT-PCR analyses revealed that CD271 mRNA expression was increased in PSCs after coculture with pancreatic cancer cells. However, the level of CD271 mRNA expressionsubsequently decreased after the transient increase. Furthermore, CD271 mRNA expression was decreased in PSCs migrating toward pancreatic cancer cells through Matrigel.Immunohistochemistry of PDAC revealed stromal CD271 high expression was associated with a good prognosis. These findings suggest that CD271+ PSCs appear at the early stage ofpancreatic carcinogenesis and have suppressive role against pancreatic cancer cells through cancer-stromal interaction