The effect of hypothermia therapy on cortical laminar disruption following ischemic injury in neonatal mice

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791603
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Yamaguchi University
• Principal Investigator: KIDA Hiroyuki 山口大学, 大学院医学系研究科, 助教 (70432739)
• Project Period (FY): 2011 – 2012
• Keywords: 脳性麻痺 / 低酸素虚血 / 低体温療法

Research Abstract

Cerebral ischemia was induced in P3 mice with a right common carotid artery ligation followed by hypoxia (6% O2, 37 °C) for 30 min. Immediately after hypoxic ischemia, mice were exposed to hypothermia or normothermia for 24 h. The laminar structure of the cortex was examined with immunohistochemistry (Cux1/Ctip2); the number of neurons was counted; and the expression of myelin basic protein (MBP) was determined. The hypothermia treatment was associated with improved neurological outcomes in the behavioral test. In the normothermia group, histological analyses indicated reduced numbers of neurons, reduced cortical laminar thickness in the deep, ischemic cortical layers, and significant reduction in MBP expression in the ischemic cortex compared to the contralateral cortex. In the hypothermia group, no reductions were noted in deep cortical layer thickness and in MBP expression in the ischemic cortex compared to the contralateral cortex. Our findings may provide a basis for testing hypothermal therapies in patients with cerebral palsy induced by hypoxic ischemia in the preterm period.

Research Products

• [Journal Article] The effect of hypothermia therapy on cortical laminar disruption following ischemic injury in neonatal mice (2013)
  • Author(s): Kida H, Nomura S, Shinoyama M, Ideguchi M, Owada Y and Suzuki M
  • Journal Title: PLOS ONE Volume: (In press)
  • Peer Reviewed
• [Journal Article] Penicillin-induced epileptiform activity elevates focal brain temperature in anesthetized rats (2013)
  • Author(s): Tokiwa T, Inoue T, Fujii M, Ishizuka S, Aou S, Kida H, Maruta Y, Yamakawa T, Nomura S, Suzuki M, Yamakawa T
  • Journal Title: Neurosci Res Volume: (In press)
  • DOI: DOI:10.1016/j.neures.2013.05.001
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of focal brain cooling on photochemically-induced cerebral infarction in rats: analysis from a neurophysiological perspective (2013)
  • Author(s): He Y, Fujii M, Inoue T, Nomura S, Maruta Y, Oka F, Shirao S, Owada Y, Kida H, Kunitsugu I, Yamakawa T, Tokiwa T, Yamakawa T, Suzuki M
  • Journal Title: BrainRes Volume: 25 Pages: 1553-60
  • DOI: DOI:10.1016/j.brainres.2012.11.041
  • Peer Reviewed
• [Journal Article] Focal brain cooling terminates the faster frequency components of epileptic discharges induced by penicillin G in anesthetized rats (2012)
  • Author(s): Kida H, Fujii M, Inoue T, He Y, Maruta Y, Nomura S, Taniguchi K, Ichikawa T, Saito T, Yamakawa T, Suzuki M.Clin
  • Journal Title: Clinical Neurophysiol Volume: 123(9) Pages: 1708-13
  • DOI: DOI:10.1016/j.clinph.2012.02.074
  • Peer Reviewed
• [Journal Article] Effects of intrathecal baclofen therapy on motor and cognitive functions in a rat model of cerebral palsy (2012)
  • Author(s): Nomura S, Kagawa Y, Kida H, Maruta Y, Imoto H, Fujii M, Suzuki M
  • Journal Title: J Neurosurg Pediatr Volume: 9(2) Pages: 209-15
  • DOI: DOI:10.3171/2011.10.PEDS11209
  • Peer Reviewed
• [Presentation] The effect of hypothermia on the laminar disruption and neural injury in neonatal mice following ischemic injury (2012)
  • Author(s): Hiroyuki Kida, Sadahiro Nomura, Mizuya Shinoyama, Makoto Ideguchi, Yuji Owada and Michiyasu Suzuki
  • Organizer: 42th. Soc Neurosci
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20121013-17
• [Presentation] Focal brain cooling suppresses periodic epileptifrom discharges and lessens brain injury after focal cerebral ischemia in rats (2012)
  • Author(s): 賀業霆、藤井正美、井上貴雄、野村貞宏、木田裕之、丸田雄一、国次一郎、山川烈、鈴木倫保
  • Organizer: 第46回日本てんかん学会(JES2012)
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 20121011-12
• [Presentation] 新生児低酸素虚血障害による低体温療法の効果の検討 (2012)
  • Author(s): 木田裕之、野村貞宏、篠山瑞也、出口誠、大和田祐二、鈴木倫保
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 20120918-20
• [Presentation] 低酸素性虚血脳障害由来マイクログリアの炎症性および抗炎症性サイトカイン産生に及ぼす軽度低温の影響 (2012)
  • Author(s): 松井智浩、伊波琢也、小原旅人、木田裕之、野村貞宏、鈴木倫保
  • Organizer: 第15回日本脳低温療法学会
  • Place of Presentation: 横浜シンポジア(横浜)
  • Year and Date: 20120706-07
• [Presentation] Challenges and Opportunities for development of a focal cooling device for intractable epilepsy (2012)
  • Author(s): Takao Inoue, Masami Fujii, Tatsuji Tokiwa, Yuichi Maruta, Hiroyuki Kida, Yeting He, Toshitaka Yamakawa, Hirochika Imoto, Sadahiro Nomura, Kenichi Hirano, Masanori Niiyama, Hiromi Yamazaki, Yuji Owada, Takeshi Yamakawa, and Michiyasu Suzuki
  • Organizer: Korean Epilepsy Congress (KEC2012)
  • Place of Presentation: Songdo Convensia, incheon, Korea
  • Year and Date: 20120607-09
• [Presentation] てんかんモデルラットにおける局所脳冷却の脳波周波数解析 (2012)
  • Author(s): 木田裕之、藤井正美、井上貴雄、賀業霆、丸田雄一、野村貞宏、谷口和博、市川琢也、斉藤俊、山川烈、鈴木倫保
  • Organizer: 第6回日本てんかん学会中国・四国地方大会
  • Place of Presentation: 高知市文化プラザかるぽーと(高知)
  • Year and Date: 2012-02-11
• [Presentation] The effect of focal brain cooling on epileptic discharges in rats: Frequency analysis of electroencephalography (2011)
  • Author(s): Hiroyuki Kida, Masami Fujii, Takao Inoue, Yeting He, Yuichi Maruta, Sadahiro Nomura, Kazuhiro Taniguchi, Takuya Ichikawa, Takashi Saito, Takeshi Yamakawa, and Michiyasu Suzuki
  • Organizer: The 2011 IEEE International Conference on Systems, Man, and Cybernetics
  • Place of Presentation: Alaska, USA
  • Year and Date: 20111009-12
• [Presentation] Development of a focal cerebral cooling system for the treatment of intractable epilepsy: An experimental study in cats and non-human primates (2011)
  • Author(s): Takao Inoue, Masami Fujii, Yeting He, Yuichi Maruta, Hiroyuki Kida, Sadahiro Nomura, Tatsuji Tokiwa, Toshitaka Yamakawa, Kenichi hirano, Masanori Niiyama, Hiromi Yamazaki, Takeshi Yamakawa, and Michiyasu Suzuki
  • Organizer: The 2011 IEEE International Conference on Systems, Man, and Cybernetics
  • Place of Presentation: Alaska, USA
  • Year and Date: 20111009-12
• [Presentation] Frequency spectrum analysis of electroencephalograms during focal brain cooling in experimental neocortical seizures (2011)
  • Author(s): Kazuhiro Taniguchi, Takuya Ichikawa, Hiroyuki Kida, Masami Fujii, Takao Inoue, Yeting He, Yuichi Maruta, Sadahiro Nomura, Michiyasu Suzuki, Takeshi Yamakawa, Takashi Saito
  • Organizer: 65th American Epilepsy Society Annual Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-12-03
• [Remarks] ホームページ
  • URL: http://ds.cc.yamaguchi-u.ac.jp/~seiri2/index.html