Effect of chondrogenic differentiation in mesenchymal stem cells on the stiffness of three dimensional extracellular matrix

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791678
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Metropolitan Industrial Technology Research Institute
• Principal Investigator: OHYABU Yoshimi 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 副主任研究員 (80587410)
• Project Period (FY): 2011-04-28 – 2014-03-31
• Keywords: 硬さ / 3次元 / ゼラチン / 線維化

Outline of Final Research Achievements

Generically, we used crosslinker that has a cytotoxic to control stiffness and was not able to be cultured for the cells in three-dimensional. Gelatin uncleaved molecular chains (uncleaved gelatin; UCG) were tends to restore the helical structure at 30 degrees C and confirmed to form fibrils similar to the collagen fibers. We identified the fiber-forming component that is included in UCG and developed a method for fabrication of high-density collagen fibril matrix gels from concentrated solutions of UCG. High-strength CFM may provide more durable hydrogels for tissue engineering and regenerative medicine.

Free Research Field

医歯薬学