The elucidation of the MRTF mediated molecular mechanism of renalinterstitial fibrosis in obstructive nephropathy

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791777
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 (20398374)
• Project Period (FY): 2011 – 2012
• Keywords: 腎線維化 / MRTF / 片側尿管閉塞マウスモデル / 上皮間葉転換

Research Abstract

We examined the effect of MRTF inhibition by MRTF siRNAs on the activation of renal interstitial fibroblasts in a rat renal interstitial fibroblast line (NRK -49F) and on the development of fibrosis in a mouse model of renal fibrosis induced by unilateral ureteral obstruction (UUO). In vitro, we confirmed the functional significance of MRTF for the maintenance of fibroblast phenotype. However, in a mouse model of UUO, we have not confirmed that MRTF siRNAs can decrease renal interstitial fibrosis yet. Because the effectiveness of MRTF siRNAs is not sufficient, we can not obtain the exact results. Recently, we have been examining the right conditions of MRTF siRNAs transfections.