2013 Fiscal Year Final Research Report

Effects of estrogen receptor expression in paracrine regulation of cell proliferation of mammary gland epithelial cell line

Research Project

Project/Area Number	23791823
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Obstetrics and gynecology
Research Institution	University of Yamanashi
Principal Investigator	ISHIDA Maho 山梨大学, 医学工学総合研究部, 助教 (80362086)
Project Period (FY)	2011 – 2012
Keywords	エストロジェン / エストロジェン受容体 / 乳腺上皮細胞 / 細胞増殖 / 傍分泌
Research Abstract	We examined the mitogenic effect of estrogen on mammary gland epithelial cells without the expression of ERalpha through paracrine factors produced by mammary ERalpha-expressing cells. Two types of cells with and without the expression of ERalhpa, both of which were derived from MCF 10A, the non-tumorigenic mammary gland epithelial cell line, were co-cultured. Treatment of cocultures with estrogen resulted in the inhibition of the ERalpha-expressing cells as demonstrated in previous studies. The fact that the proliferation of the cells without the expression of ERalpha was not changed by the treatment with estrogen indicates the absence of the mitogenic effect of estrogen through the paracrine mechanism in this system.