2013 Fiscal Year Final Research Report

A prediction and a therapeutic strategy for lung hypoplasia in a rat CDH model

Research Project

Project/Area Number	23791826
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Obstetrics and gynecology
Research Institution	Nagoya University
Principal Investigator	TSUDA HIROYUKI 名古屋大学, 医学部附属病院, 助教 (40571328)
Project Period (FY)	2011 – 2013
Keywords	横隔膜ヘルニア / 肺低形成 / ラメラ体 / 羊水
Research Abstract	We initially enrolled 19 cases diagnosed with CDH prenatally. LBC was significantly higher in the surviving group than in deceased group (p < 0.01).In the rat experiment, we collected amniotic fluid and lung tissue from newborns at E21 and confirmed the presence of lamellar bodies in both the amniotic fluid. We also confirmed a similar lamellar body size in amniotic fluid and type II alveolar cells comparable to the lamellar body findings in human amniotic fluid. Next, after exposure of pregnant rats to a 100 mg dose of nitrofen, 48.8% (61/125) of newborns had diaphragmatic hernias. LBC was significantly higher in control than in CDH (p < 0.01) rats. Furthermore, lung weight was also significantly higher in control than in CDH (p < 0.01) newborn rats. We examined the expression of ABCA3 in lung tissues from newborn rats, delivered at E21, by Western blotting. ABCA3 expression did not differ significantly between controls and rats with CDH.