2012 Fiscal Year Final Research Report
Elucidation of the variance of intercellular phosphorylation signaling in hair cells during inner ear disorder
| Project/Area Number |
23791873
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経科学 / 内耳障害 / チロシンリン酸化 / シグナル伝達 |
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| Research Abstract |
To evaluate phosphorylation state of proteins present in the inner ear organ, we used inner ear organ homogenate from wild mice. Immunoblot analysis indicated that several proteins including cochlin undergo tyrosine phosphorylation with pervanadate stimulation. We investigated the phosphorylation state about full length form of cochlin in pervanadate stimulation with immnunoprecipitation method, and the immnunoprecipitated cochlin protein was indeed tyrosine phosphorylated.
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