2013 Fiscal Year Final Research Report
Phosphatidylcholine Species in the endolymphatic hydrops by Mass Microscopy
Project/Area Number |
23791890
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
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Keywords | 脂質 / 内耳 |
Research Abstract |
We recently developed a mass microscope consisting of a mass spectrometry imager with high spatial resolution equipped with an atmospheric pressure matrix-assisted laser desorption/ionization and quadrupole ion trap time-of-flight analyzer. Phosphatidylcholine (PC), a phospholipid, is a basic structural component of cell membranes. The distributions of PC species in the inner ear have not been fully understood. In this study, we extended the use of our mass microscope to the mouse inner ear with special attention to the vestibular organ. We clearly localized some PC species in the vestibular organ and cochlea. PC (16:0/20:4) was observed mainly in the vestibular sensory cells and PC (16:0/18:1) was highly localized in the nerve fiber under sensory cells. PC (16:0/16:1) was found primarily in the organ of Corti. These distributional differences may be associated with the cellular architecture of these inner ear regions.
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Research Products
(8 results)
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[Presentation] Clinical Study of Mucosa-Associated Lymphoid Tissue(MALT) Lymphomas of the Head and Neck2012
Author(s)
Sugiyama K, Hosokawa S, Mochizuki D, Okamura J, Takizawa Y, Takahashi G, Hosokawa K, Yamatodani T, Misawa K, Mineta H
Organizer
American Head & Neck Society 2012 8th International Conference
Place of Presentation
Toronto, Ontario, Canada
Year and Date
20120721-25
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[Presentation] 質量顕微鏡による内耳の解析2012
Author(s)
杉山健一,瀧澤義徳,水田邦博,大和谷崇,泉智沙子,細川久美子,中西啓,望月大極,早坂孝宏,瀬藤光利,峯田周幸
Organizer
第113回日本耳鼻咽喉科学会総会
Place of Presentation
新潟
Year and Date
2012-05-10
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[Presentation] 質量顕微鏡による5種類のホスファチジルコリンの蝸牛における局在2011
Author(s)
瀧澤義徳,水田邦博,中西啓,橋本泰幸,大和谷崇,岡村純,杉山健一,木村桃子,鈴木高広,早坂孝宏,瀬藤光利,峯田周幸
Organizer
第112回日本耳鼻咽喉科学会総会
Place of Presentation
京都
Year and Date
2011-05-21