Phosphatidylcholine Species in the endolymphatic hydrops by Mass Microscopy

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791890
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: SUGIYAMA Kenichi 浜松医科大学, 医学部附属病院, 助教 (50402334)
• Project Period (FY): 2011 – 2012
• Keywords: 脂質 / 内耳

Research Abstract

We recently developed a mass microscope consisting of a mass spectrometry imager with high spatial resolution equipped with an atmospheric pressure matrix-assisted laser desorption/ionization and quadrupole ion trap time-of-flight analyzer. Phosphatidylcholine (PC), a phospholipid, is a basic structural component of cell membranes. The distributions of PC species in the inner ear have not been fully understood. In this study, we extended the use of our mass microscope to the mouse inner ear with special attention to the vestibular organ. We clearly localized some PC species in the vestibular organ and cochlea. PC (16:0/20:4) was observed mainly in the vestibular sensory cells and PC (16:0/18:1) was highly localized in the nerve fiber under sensory cells. PC (16:0/16:1) was found primarily in the organ of Corti. These distributional differences may be associated with the cellular architecture of these inner ear regions.

Research Products

• [Journal Article] Relationship between tympanic membrane retraction and habitual sniffing in patients with cholesteatoma (2013)
  • Author(s): Takizawa Y, Mizuta K, Hamada N, Yamatodani T, Nakanishi H, Hosokawa K, Takahashi G, Sugiyama K, Mineta H
  • Journal Title: Acta Otolaryngol Volume: 133(10) Pages: 1030-1034
  • DOI: 10.3109/00016489.2013.808763
• [Journal Article] Congenital middle ear cholesteatoma : experience from 26 surgical cases (2013)
  • Author(s): Yamatodani T, Mizuta K, Hosokawa K, Takizawa Y, Sugiyama K, Nakanishi H, Mineta H
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 122(5) Pages: 316-21
• [Journal Article] ラット前庭器官の暗細胞におけるG蛋白Gsの電顕的局在(Ultrastructural Localization of the G-protein Gs in the Dark Cells of Rat Vestibular Organs.) (2013)
  • Author(s): Kunihiro Mizuta, Yoshinori Takizawa, Kenichi Sugiyama, Seiji Hosokawa, Hiroshi Nakanishi, Maki Arai, Masahiko Adachi, Hiroyuki Mineta
  • Journal Title: 耳鼻咽喉科臨床補冊 Volume: 136 Pages: 58-61
• [Presentation] Phosphatidylcholine species localization in the mouse inner ear by mass microscopy (2013)
  • Author(s): Takizawa Y, Mizuta K, Sugiyama K, Hayasaka T, Nakanishi H, Okamura J, Mineta H, Setou M
  • Organizer: The 36th Annual mid-winter research meeting of the association for research of otolaryngology
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 20130216-20
• [Presentation] Clinical Study of Mucosa-Associated Lymphoid Tissue(MALT) Lymphomas of the Head and Neck (2012)
  • Author(s): Sugiyama K, Hosokawa S, Mochizuki D, Okamura J, Takizawa Y, Takahashi G, Hosokawa K, Yamatodani T, Misawa K, Mineta H
  • Organizer: American Head & Neck Society 2012 8th International Conference
  • Place of Presentation: Toronto, Ontario, Canada
  • Year and Date: 20120721-25
• [Presentation] Relationship between the findings of tympanic membrane and the habit of sniffing among cholesteatoma (2012)
  • Author(s): Takizawa Y, Mizuta K, Yamatodani T, Hosokawa K, Nakanishi H, Takahashi G, Sugiyama K, Okamura J, Mineta H
  • Organizer: The 9th International Conference on Cholesteatoma and Ear Surgery
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 20120603-07
• [Presentation] 質量顕微鏡による内耳の解析 (2012)
  • Author(s): 杉山健一,瀧澤義徳,水田邦博,大和谷崇,泉智沙子,細川久美子,中西啓,望月大極,早坂孝宏,瀬藤光利,峯田周幸
  • Organizer: 第113回日本耳鼻咽喉科学会総会
  • Place of Presentation: 新潟
  • Year and Date: 2012-05-10
• [Presentation] 質量顕微鏡による5種類のホスファチジルコリンの蝸牛における局在 (2011)
  • Author(s): 瀧澤義徳,水田邦博,中西啓,橋本泰幸,大和谷崇,岡村純,杉山健一,木村桃子,鈴木高広,早坂孝宏,瀬藤光利,峯田周幸
  • Organizer: 第112回日本耳鼻咽喉科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-05-21