2012 Fiscal Year Final Research Report
Phosphorylcholine Suppresses the AllergicRhinitis inMice
Project/Area Number |
23791911
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
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Research Institution | Kagoshima University |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
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Keywords | 粘膜ワクチン / Th1/Th2バランス / Phosphprylcholine / アレルギー |
Research Abstract |
Background: Phosphorylcholine (PC), which is a structural component of wide variety of bacteria. However immunomodulatory properties of PC in the upper respiratory tract have not yet been characterized. Objective: We sought to elucidate the effects of PC on the regulation of allergic response in the upper respiratory tract. Methods: Allergic rhinitis model established by ovalbumin (OVA) sensitization was pretreated by intranasal or peritoneal administrationof PC-bovine serum albumin (PC-BSA). Allergic responses were compared with non-pretreated mice. IL-12p40 production by DCs stimulated with PC was measured to evaluate the effect of PC on the TH2 responses mediated by DCs. Results: PC-pretreated mice showed mild allergic nasal symptoms compared to the mice without PC-pretreatment. The levels of total IgE and OVA-specific IgE were significantly lower inPC-pretreated mice. The IL-4 production by splenic CD4+T cells was down-regulated by PC-pretreatment. Further, PC induced IL-12p40 production by DCs. Conclusion: These findings indicate that PC inhibits the allergic response in the upper respiratory tract mediated by the induction of IL-12p40 production by DCs. Thus, PC might be useful as an immunotherapeutic agent for allergic diseases.
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