2012 Fiscal Year Final Research Report

Breakthrough of the pathogenesis and treatment strategy in necrotizing enterocolitis using two-photon laser scanning microscopy based on the alarmin concept.

Research Project

Project/Area Number	23792027
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Pediatric surgery
Research Institution	Mie University
Principal Investigator	KOIKE Yuhki 三重大学, 大学院・医学系研究科, 助教 (10555551)
Project Period (FY)	2011 – 2012
Keywords	先天性消化器疾患学 / two-photon laser-scanning microscopy (TPLSM) / necrotizing enterocolitis (NEC) model
Research Abstract	This research project is making the necrotizing enterocolitis model created in beta-actin-green fluorescent protein transgenic mice observed by two-photon laser-scanning microscopy(TPLSM) , and figuring out the pathogenesis and treatment of this disease using this model and system. As for the preliminary study, we performed the LPS induced septic model mouse, and achieved the observation of dynamic pathology of intestinal microcirculation in this septic model mouse. Nest stage, we tried to performed the necrotizing enterocolitis (NEC) model in neonatal mouse induced by LPS with hypoxia (with or without hypothermia exposure).

(3 results)