Apoptotic cell engulfment by cancer cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: YAMAZAKI Manabu 新潟大学, 医歯学系, 助教 (10547516)
• Project Period (FY): 2011 – 2012
• Keywords: 癌 / 病理学 / アポトーシス

Research Abstract

Apoptotic oral squamous cell carcinoma (SCC) cells are engulfed not only by macrophages but also by neighboring SCC cells, which could be a potential anti-cancer avenue. Since apoptotic SCC cells were immunohistochemically positive for milk fat globule-epidermal growth factor-factor 8 (MFG-E8), one of the phagocytosis regulating molecules, apoptotic SCC cells were suggested to be removed by the MFG-E8-related phagocytotic pathways. In addition, MFG-E8 was simultaneously expressed in SCC cells forming small foci at the invading front, and its expression levels in SCC cells in culture were correlated with cell growth, anti-apoptotic activity and invasion. The results indicated that the apoptotic cell clearance via MFG-E8 produced by cancer cells enhances aggressiveness of cancer cell behaviors.

Research Products

• [Journal Article] Hemophagocytosis-mediated keratinization in oral carcinoma in-situ and squamous cell carcinoma: a possible histophathogenesis of keratin pearls (2013)
  • Author(s): Al-Eryani K, Cheng J, Abe T, Yamazaki M, Maruyama S, Tsuneki M, Essa A, Babkair H, Saku T
  • Journal Title: J Cell Physiol Volume: (in press)
  • DOI: DOI:10.1002/jcp.24364
  • Peer Reviewed
• [Journal Article] Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinomacells (2013)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Xu B, Essa A, Abe T, Babkair H, Cheng J,Yamamoto T, Saku T
  • Journal Title: Biochem Biophys Res Commun Volume: 434(1) Pages: 12-130
  • DOI: DOI:10.1016/j.bbrc.2013.03.057
  • Peer Reviewed
• [Journal Article] Podoplanin is a novel myoepithelial cell marker in pleomorphic adenoma and other salivary gland tumors with myoepithelialdifferentiation (2013)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Essa A, Abe T, Babkair HA, Ahsan MS, Cheng J, Saku T
  • Journal Title: Virchows Arch Volume: 462(3) Pages: 297-305
  • DOI: DOI:10.1007/s00428-012-1359-z
  • Peer Reviewed
• [Journal Article] Inflammatory histopathogenesis of nasopalatine duct cyst: a clinicopathological study of 41 cases (2013)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Abe T, Adeola H, Cheng J, Nishiyama H, Hayashi T, Kobayashi T, Takagi R, Funayama A, Saito C, Saku T
  • Journal Title: Oral Dis Volume: 19(4) Pages: 415-424
  • DOI: DOI:10.1111/odi.12022
  • Peer Reviewed
• [Journal Article] Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma (2012)
  • Author(s): Metwaly H, Maruyama S, Yamazaki M, Tsuneki M, Abe T, Jen KY, Cheng J, Saku T
  • Journal Title: Hum Pathol Volume: 43(11) Pages: 1973-1981
  • DOI: DOI:10.1016/j.humpath.2012.02.006
  • Peer Reviewed
• [Journal Article] Clinical significance of KRAS gene mutationand epidermal growth factor receptor expression in Japanese patients with squamous cell carcinoma of the larynx, oropharynx and hypopharynx (2012)
  • Author(s): Fujii S, Uryu H, Akashi K, Suzuki K, Yamazaki M, Tahara M, Hayashi R, Ochiai A
  • Journal Title: Int J Clin Oncol
  • DOI: DOI:10.1007/s10147-012-0402-z
  • Peer Reviewed
• [Journal Article] Intraepithelially entrapped blood vessels in oral carcinoma in-situ (2012)
  • Author(s): Funayama A, Maruyama S, Yamazaki M, Al-Eryani K, Shingaki S, Saito C, Cheng J, Saku T
  • Journal Title: Virchows Arch Volume: 450 Pages: 473-480
  • DOI: DOI:10.1007/s00428-012-1224-0
  • Peer Reviewed
• [Journal Article] Podoplanin expression profiles characteristic of odontogenic tumor-specific tissue architectures (2012)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Cheng J, Saku T
  • Journal Title: PatholRes Pract Volume: 208 Pages: 140-146
  • DOI: DOI:10.1016/j.prp.2011.12.016
  • Peer Reviewed
• [Journal Article] Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections (2012)
  • Author(s): Ida-Yonemochi H, Maruyama S, Kobayashi T, Yamazaki M, Cheng J, Saku T
  • Journal Title: Mod Pathol Volume: 25 Pages: 784-794
  • DOI: DOI:10.1111/j.1365-2559.2010.03732.x
  • Peer Reviewed
• [Journal Article] Enhanced expression of podoplanin in oral carcinoma in situ and squamous cellcarcinomas (2011)
  • Author(s): Funayama A, Cheng J, Maruyama S, Yamazaki M, Kobayashi T, Syafriadi M, Kundu S, Shingaki S, Saito C, Saku T
  • Journal Title: Pathobiology Volume: 78 Pages: 171-180
  • DOI: DOI:10.1159/000324926
  • Peer Reviewed
• [Journal Article] Emergence of keratin 17 vs. loss of keration 13: their reciprocal immunohistochemical profiles in oral carcinoma in situ (2011)
  • Author(s): Mikami T, Cheng J, Maruyama S, Kobayashi T, Funayama A, Yamazaki M, Adeola HA, Wu L, Shingaki S, Saito C, Saku T
  • Journal Title: Oral Oncol Volume: 47 Pages: 497-503
  • DOI: DOI:10.1016/j.oraloncology.2011.03.015
  • Peer Reviewed
• [Journal Article] Nuclear translocation of β-catenin synchronized with loss ofE-cadherin in oral epithelial dysplasia with a characteristic two-phase appearance (2011)
  • Author(s): Alvarado CG, Maruyama S, Cheng J,Ida-Yonemochi H, Kobayashi T, Yamazaki M, Takagi R, Saku T
  • Journal Title: Histopathology Volume: 59 Pages: 283-291
  • DOI: DOI:10.1111/j.1365-2559.2011.03929.x
  • Peer Reviewed
• [Journal Article] Differential expression of perlecan receptors, α-dystroglycan and integrin β1, before and after invasion of oral squamous cell carcinoma (2011)
  • Author(s): Ahsan MS, Yamazaki M, Maruyama S, Kobayashi T, Ida-Yonemochi H, Hasegawa M, Henry Ademola A, Cheng J, Saku T
  • Journal Title: J Oral Pathol Med Volume: 40 Pages: 552-559
  • DOI: DOI:10.1111/j.1600-0714.2010.00990.x
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌における同種間アポトーシス細胞処理機構とその意義 (2012)
  • Author(s): 山崎学
  • Organizer: 第23回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 東京都
  • Year and Date: 20120829-31
• [Presentation] MFG-E8 promotes apoptotic cell clearance by cancer cells and cancer progression in oralsquamous cell carcinoma (2011)
  • Author(s): 山崎学,丸山智,程〓,朔敬
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 20111003-05
• [Presentation] 下顎腫瘍 (2011)
  • Author(s): 山崎学,丸山智,程〓,藤田一,高木律男,林孝文,朔敬
  • Organizer: 第22回日本臨床口腔病理学会総会・学術大会・第5回アジア口腔病理学会
  • Place of Presentation: 福岡市
  • Year and Date: 20110823-25
• [Presentation] 口腔扁平上皮癌におけるMFG-E8の発現:腫瘍進展とアポトーシス細胞処理への関与 (2011)
  • Author(s): 山崎学,丸山智,程〓,朔敬
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 20110428-30