2012 Fiscal Year Final Research Report
Characterization of myoblast differentiation and localization during early myogenesis in mouse tongue
| Project/Area Number |
23792116
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
FUJITA Kazuya 日本歯科大学, 生命歯学部, 助教 (70549055)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 歯学 / マウス胎仔 / 舌初期発生 / 筋芽細胞分化 / マイクロアレイ / IPA 解析 |
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| Research Abstract |
The present study aimed to characterize the molecular networks that govern tongue myogenesis in mouse embryos. The gene expression profiling by microarray analyses, together with quantitative PCR analysis and immunohistochemical validation, demonstrated that the expression of myogenesis-related genes were strongly up-regulated during early tongue morphogenesis (Embryonic days from 10.5 to 11.5). We also delineated concomitant expression of microRNAs that might interact with transcripts of regulatory genes for myoblast differentiation in tongue primordium.
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