Roles of a heretical Wnt receptor, Ryk for bone remodeling

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
• Project Period (FY): 2011 – 2012
• Keywords: 骨代謝 / Wnt, Ryk, 骨芽細胞 / 破骨細胞

Research Abstract

The purpose of this study is clarifying a role of atypical Wnt receptor Ryk in the bone metabolism. Ryk was called a heretical Wnt receptor because of its function and properties in both canonical and non-canonical Wnt pathway. Ryk binds to Wnt3a (a canonical ligand) and Wnt5a (a non-canonical ligand), and transduces signals to control many biological processes. Ryk is expressed in both osteoclast-lineage cells and osteoblasts. Wegenerated conditional Ryk knockout mice to delete Ryk gene in these cells.

Research Products

• [Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL-34 expression in osteopetrotic op/op mice. (2012)
  • Author(s): Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N.;
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 10006-1
  • DOI: DOI:10.1073/pnas.1207361109.
  • Peer Reviewed
• [Journal Article] Fos plays an essential role in the upregulation of RANK expression in osteoclast precursors within the bone microenvironment. (2012)
  • Author(s): Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger JM, Yamada K, Udagawa N, Takahashi N.;
  • Journal Title: J Cell Sci Volume: 125 Pages: 2910-75
  • DOI: DOI:10.1242/jcs.099986.
  • Peer Reviewed
• [Journal Article] Daily administration of eldecalcitol (ED-71), an active vitamin D analog, increases bone mineral density by suppressing RANKL expression in mouse trabecular bone. (2012)
  • Author(s): Harada S, Mizoguchi T, Kobayashi Y, Nakamichi Y, Takeda S, Sakai S, Takahashi F, Saito H, Yasuda H, Udagawa N, Suda T, Takahashi N
  • Journal Title: J Bone Miner Res Volume: 27 Pages: 461-73
  • DOI: DOI:10.1002/jbmr.555.
  • Peer Reviewed
• [Journal Article] Lineage-committed osteoclast precursors circulate in blood and settle down into bone. (2011)
  • Author(s): Muto A, Mizoguchi T, Udagawa N, Ito S, Kawahara I, Abiko Y, Arai A, Harada S, Kobayashi Y, Nakamichi Y, Penninger JM, Noguchi T, Takahashi N.;
  • Journal Title: J Bone Miner Res Volume: 26 Pages: 2978-90
  • DOI: DOI:10.1002/jbmr.490.
  • Peer Reviewed
• [Journal Article] 破骨細胞前駆細胞の形成と供 給におけるビタミン D の役割の新知見 (2011)
  • Author(s): 中道裕子
  • Journal Title: THE BONE Volume: 25 Pages: 233-237
  • URL: http://www.de-hon.ne.jp/digital/tmp/4A03DDAE2E9E40899044B70D623658FC/2000003264009800600P0B.pdf
• [Presentation] 破骨細胞形成におけるM-CSF/IL-34/RANKL のそれぞれの役割」 (2012)
  • Author(s): 中道裕子 ;
  • Organizer: 第15回骨発生・再生研究会
  • Place of Presentation: 慶応大学(東京都新宿区)
  • Year and Date: 2012-11-10
• [Presentation] M-CSF 欠損 op/op マウスに おいて,脾臓は破骨細胞前駆細胞の貯蔵 庫として機能する:破骨細胞形成におけるIL-34 の新しい役割 (2012)
  • Author(s): 中道裕子 ,溝口利英,荒井敦,小林泰浩, 保田尚孝,加藤茂明,須田立雄,宇田川信 之,高橋直之
  • Organizer: 日本骨代謝学会学術 集会 (第30回)
  • Place of Presentation: 京王プ ラザホテル(東京都新宿区)
  • Year and Date: 2012-07-21
• [Presentation] Spleen serves as a reservoir of osteoclast precursors through IL-34 expression in op/op mice (2012)
  • Author(s): Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Penninger JM, Yasuda H, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N
  • Organizer: 4th InternationalConference on Osteoimmunology(第4回国際骨免疫学会議)
  • Place of Presentation: Chandris Hotel(コルフ島,ギリシア)
  • Year and Date: 2012-06-21
• [Presentation] 破骨細胞形成におけるM-CSF/RANKL/IL-34のそれぞれの役割 (2012)
  • Author(s): 中道裕子,荒井敦,小林泰浩,宇田川信之,高橋直之;
  • Organizer: 松本歯科大学学会例会(第75回)
  • Place of Presentation: 松本歯科大学(長野県塩尻市
  • Year and Date: 2012-01-21
• [Presentation] Dental Pulp Cells Exhibit High Performance as a Biomaterial for Bone Formation Through Expression of Annexin A8 (2011)
  • Author(s): Nakamichi Y, Hagihara T, Nakamura M, Imaoka A, Abiko Y, Nakamura H, Takahashi N, Udagawa N.
  • Organizer: 第31回米国骨代謝学会
  • Place of Presentation: サンディエゴコンベンションセンター(カリフォルニア州,米国)
  • Year and Date: 2011-09-17
• [Presentation] 歯髄細胞の有する顕著な骨再生能力は、annexin A8を介して発揮される (2011)
  • Author(s): 中道裕子,萩原貴寛,中村美どり,高橋直之,宇田川信之
  • Organizer: 日本骨代謝学会 (第29回)
  • Place of Presentation: 大阪国際会議場(大阪市北区)
  • Year and Date: 2011-07-30