Analysis of infection control on endodontic treatment using genetic engineering method

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792172
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: NAGAYAMA Tomotaka 大阪大学, 大学院・歯学研究科, 招聘教員 (60456944)
• Project Period (FY): 2011 – 2012
• Keywords: 歯学 / 歯内療法学 / 細菌 / バイオフィルム / 術中感染 / ラバーダム防湿 / 16S rRNA遺伝子解析法

Research Abstract

To clarify the precise condition of infection control on endodontic treatment, bacterial biofilm in root canal before or after treatment, which was classified into 4 groups (pulpectomy or infected root canal treatment, with or without rubber dam isolation technique), was analyzed by PCR-based 16S rRNA gene assay. Furthermore, patient’s saliva, dental plaque and glove was analyzed similarly. Although it was difficult to clarity the route of infection completely, we demonstrated the usefulness of rubber dam isolation technique for infection control on endodontic treatment.

Research Products

• [Journal Article] う蝕象牙質除去後の残存細菌に Er:YAG レーザーが与える影響 (2013)
  • Author(s): 高橋雄介,吉岡靖介,朝日陽子,永山智崇,野杁由一郎,林美加子
  • Journal Title: 日歯科保誌 Volume: 56(1) Pages: 1-8
  • Peer Reviewed
• [Presentation] 根尖孔外バイオフィルムモデルにおけるラット根尖病巣のマイクロ CT 解析 (2012)
  • Author(s): 呉本勝隆,野杁由一郎,永山智崇,騎馬和歌子,前薗葉月,山本れいこ,米田直道,林美加子,恵比須繁之,石本卓也,中野貴由
  • Organizer: 第137回秋季日本歯科保存学会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-23
• [Presentation] 難治性根尖性歯周炎に関わるバイオフィルム構成細菌種と臨床症状との関係 (2012)
  • Author(s): 藪根敏晃,野杁由一郎,山本れいこ,山口幹代,朝日陽子,前薗葉月,永山智崇,呉本勝隆,騎馬和歌子,林美加子,恵比須繁之
  • Organizer: 第136回春季日本歯科保存学会
  • Place of Presentation: 沖縄
  • Year and Date: 2012-06-29
• [Presentation] 軟化象牙質除去後の残存細菌に対してEr:YAG レーザーが与える影響 (2012)
  • Author(s): 吉岡靖介,野杁由一郎,高橋雄介,藪根敏晃,朝日陽子,永山智崇,古谷優,栗本絵里子,北川蘭奈,武田侑子,大嶋淳,山本由美子,住岡龍一,永井真澄,板東秀典,林美加子
  • Organizer: 第136回春季日本歯科保存学会
  • Place of Presentation: 沖縄
  • Year and Date: 2012-06-29