Elucidation of dental pulp cell differentiation by laser

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: TAKEUCHI Osamu 大阪歯科大学, 歯学部, 助教 (70548320)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞・組織 / 炎症 / レーザー / 再生医学 / シグナル伝達

Research Abstract

Dental pulp inflammation associated with the progression of dental caries facilitates the production of inflammatory cytokines, such as interleukin-1b (IL-1b), and enzymes, such as matrix metalloproteinases (MMPs), in the pulp tissue, resulting in tissue destruction. The results of this experiment suggest that MEK1/2 and ERK1/2 pathways were involved in the MMP-3 production enhanced by IL-1b stimulation in HPFs.
These samples consisted of 25 experimental group samples with 15 and 30-second, and 1, 3, and 5-minute irradiation times, and 0.5, 1, 2, 5, and 10-W irradiation outputs, and one control group sample without irradiation.However, for 0.5 - 10-W high-energy Semiconductor lasers, a decrease in the cell number due to cell death was observed after 3-minute irradiation, and no cell adhesion to the plate surface immediately after cell seeding was noted after 5-minute irradiation with the 10-W laser.

Research Products

• [Journal Article] ヒト歯髄由来線維芽細胞におけるIL-1β刺激によるMMP-3産生の影響 (2014)
  • Author(s): 竹内摂,合田征司,吉川一志,堂前英資,池尾隆,山本一世
  • Journal Title: 日本歯科保存学雑誌 Volume: 57巻 Pages: 1-8
  • Peer Reviewed
• [Journal Article] The Effect of lysenin on the differentiation into osteoclast cells (2011)
  • Author(s): Domae E, Ogawa Y, Takeuchi O, Ujii Y, Kato Y, Kawasaki T, Hayashi H, Komasa R, Domae N, Goda S, Ikeo T
  • Journal Title: J Oral Tissue Engin Volume: 9巻 Pages: 3-9
  • Peer Reviewed
• [Journal Article] Effect of GaAlAs semiconductor laser irradiation on the permeability of dentin and survival of dental pulp cells (2011)
  • Author(s): Tanaka Y, Takeuchi O, Goda S, Yoshikawa K, Yamamoto K
  • Journal Title: J Osaka Dent Univ Volume: 45巻 Pages: 7-16
  • Peer Reviewed
• [Presentation] ヒト歯髄由来線維芽細胞におけるMMP-3産生に及ぼすsmall G proteinの影響 (2013)
  • Author(s): 小正玲子,合田征司,吉川一志,竹内摂,堂前英資,三木秀治,小正紀子,池尾隆,山本一世
  • Organizer: 第139回日本歯科保存学会秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館(アトリオン)(秋田市)
  • Year and Date: 2013-10-17
• [Presentation] ヒト歯髄由来線維芽細胞におけるMMPs産生におよぼすsmall G proteinの影響 (2013)
  • Author(s): 小正玲子,竹内摂,合田征司,堂前英資,谷本啓彰,三木修治,野村雄司,池尾隆,山本一世
  • Organizer: 日本歯科保存学会2013年度春季学術大会(第138回)
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2013-06-28
• [Presentation] ヒト歯髄由来線維芽細胞におけるIL-1β刺激によるMMP-3産生への影響 (2012)
  • Author(s): 竹内摂,合田征司,小正玲子,宮地秀彦,松田有之,小松首人,藤原秀樹,池尾隆,山本一世
  • Organizer: 日本歯科保存学会2012年度秋季学術大会(第137回)
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2012-11-22
• [Presentation] 半導体レーザーが歯髄細胞に及ぼす影響について (2012)
  • Author(s): 吉川一志,竹内摂,田中芳人,鈴木康一郎,保尾謙三,廣田陽平,魯灵,畑下芳史,三木秀治,山本一世
  • Organizer: 日本歯科保存学会2012年度秋季学術大会(第137回)
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2012-11-22
• [Presentation] 二酸化チタンを併用した歯の漂白システムによる漂白効果の検討 (2012)
  • Author(s): 魯灵,小正玲子,黄地智子,廣田陽平,恩田康平,竹内摂,西田尚敬,鈴木康一郎,山本一世
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2012-11-10
• [Presentation] Application of various lining materials to dental hard tissues irradiated by Er : YAG laser (2012)
  • Author(s): Yasuo K, Yokota K, Matsuda T, Takeuchi O, Iwata N, Hattori Y, Yoshikawa K, Yamamoto K
  • Organizer: 2012 Sino- Japan Dental Conference
  • Place of Presentation: Chengdu,China
  • Year and Date: 2012-04-27
• [Presentation] ヒト歯髄由来線維芽細胞のMMP-3産生に及ぼすMAP kinaseの影響 (2011)
  • Author(s): 加藤侑,合田征司,小正玲子,竹内摂,山本一世,池尾隆,林宏行
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 20111020-21
• [Presentation] PDGF-bbはヒト歯肉由来線維芽細胞においてMMP-1産生を増強する (2011)
  • Author(s): 川崎俊也,合田征司,竹内摂,氏井庸介,林寛,小正玲子,加藤侑,林宏行,山本一世,池尾隆,松本尚之
  • Organizer: 第9回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2011-09-10